伊贝母组织培养中体细胞胚的形成及细胞组织学观察

SOMATIC EMBRYOGENESIS FROM TISSUE CULTURE OF Fritiilariae pallidiflorae AND CYTOHISTOLOGICAL OBSERVATION

伊贝母(Fritittariae pattidiftorae)鳞茎切块培养在附加NAA1.0毫克/升+KT0.5毫克/升或NAA1.0毫克/升+6—BA2.0毫克/升的MS培养基上可诱导产生胚性愈伤组织。经0—4℃低温处理4周转移到附加IAA0.1毫克/升+KT0.5毫克/升的MS培养基上30天后,通过体细胞胚途径形成了再生植株。在胚性愈伤组织分化过程中进行细胞组织学观察,发现了从单个胚性细胞到完整体细胞胚发育的各个阶段,说明伊贝母体细胞胚来源于单个胚性细胞。再生植株的根尖压片表明染色体数稳定为2n=2x=24,

Embryogenetic calli were induced from bulbs of Frutiuariae pa(?)difioia cultured on MS medium supplemented with NAA(1.0mg/1)+KT(0.5mg/1) or NAA(1.0mg/1)+6-BA(2.0mg/1), Through treating with a low temparature(0-4℃) for 4 weeks and transferring to MS medium supplemented with IAA(0.1mg/1)+KT(0.5mg/1), the calli could give rise to regenerated plantlets via somatic embryogenesis after 30 days. Cytohistologieal observations of differentiation of embryogenetic callus have found all stages of somatic embryo development, from a single embryogenic cell to an advanced stage of an embryo. It indicated that somatic embryo fo Fritiuariae pa(?)idifiorae originated from a single embryogenic cell. Root-tip squashes showed that the chromosome number of regenerated plantlets was 2n=2x=24.