摘要
观察内皮细胞在高糖环境及经肿瘤坏死因子α(TNFα)诱导后的细胞凋亡情况,并加入自由基清除剂MCI186予以干扰。检测细胞DNA裂解片段及凋亡信号蛋白(caspase)的表达量,以期探讨MCI186对高糖环境中血管内皮细胞凋亡的干预作用及凋亡机制。发现高糖环境可增加内皮细胞凋亡率,并上调TNFα诱导的调亡。MCI186可抑制高糖诱导的细胞凋亡。但无法干预高糖环境中TNFa诱导的凋亡。
In the present study, the role of a free radical scavenger (MCI-186) in preventing endothelial cell apoptosis induced by high glucose condition was studied. HDMEC were incubated with high glucose medium, with or without MCI-186. After 7 days of incubation, cell apoptosis with or without TNF-alpha was detected through DNA fragment and caspase assay. High glucose condition could enhance cell apoptosis and up-regulate TNF-alpha induced cell apoptosis. Based on the data of caspase-8 and caspase-3, TNF-alpha induced EC apoptosis might occur through mitochondria death pathway dominantly on high glucose condition. MCI-186 might suppress high glucose-induced cell apoptosis, however, it did not alter TNF-alpha induced apoptosis. These results suggest that MCI-186 may be useful in preventing endothelial cell damages by high glucose condition.
基金
国家重点研究发展规划项目资助(G1999054205)
