膀胱乳头状肿瘤DNA含量测定的研究

A study on determination of DNA content of papillary tumors of urinary bladder

应用流式细胞仪对７例膀胱乳头状瘤、２７例乳头状癌的ＤＮＡ指数和细胞增殖周期的分布进行分析，以探讨膀胱乳头状肿瘤的ＤＮＡ指数、细胞增殖周期与病理组织学的关系。其结果显示：７例乳头状瘤均为ＤＮＡ二倍体，２７例乳头状癌中５例为ＤＮ１Ａ二倍体，２２例为ＤＮＡ异倍体。ＤＮＡ含量和Ｓ＋Ｇ２Ｍ期细胞的比率与肿瘤的良恶性及恶性程度密切相关。因此，本研究结果提示，应用流式细胞仪分析肿瘤细胞的ＤＮＡ含量和细胞增殖周期的分布，可作为膀胱乳头状肿瘤的一个生物学辅助诊断指标。

in order to analyze the relationships among DNA index, cytoproliferation cycle's distribution and pathohistology of the papillary tumors of urinary bladder, flow cytometric determintiations of DNA index and distribution of cyto proliferation cycle in 7 cases of papilloma and 27 cases of papillary carcinoma of bladder were carried out. All cells of the papillomas were DNA diploidy. Of 27 cases of papillary carcinomnia, 5 were DNA diploidy and the remaining 22 were aneuploidy. The DNA content and the proportion of cells at S+G2M stages were closely related to the benign or malignant nature of the tumor and degree of malignancy. The results demonstrate that the analyses of the DNA content of tumor cells and the distribution of the cytoproliferation cycle provided by flow cytometry can be used as the biological assistant criteria for diagnosis.