摘要
目的:建立氯胺酮的薄层层析法(TLC)和气相色谱质谱(GC-MS)测定方法。方法:样品在pH13条件下用醋酸乙酯萃取,SKF525A为内标,以DB5MS石英毛细管柱、EI源、不分流进样GCMS法测定氯胺酮药物;斑点颜色结合比移值和光密度扫描图TLC法定性分析吗啡、海洛因和氯胺酮。结果:TLC法中氯胺酮与吗啡、海洛因分离良好。GCMS分析中,氯胺酮药物血药浓度在0.5~20.0mg·L$C1范围内呈线性关系,r=0.9987,最低检出限为0.2mg·L$C1(S/N=3)。结论:建立的TLC和GCMS法可用于毒品中氯胺酮的检验和氯胺酮中毒的法医学鉴定。
OBJECTIVETo establish two methods for determining the ketamin by TLC and GC-MS. METHODSThe specimens were alkalized and extracted with ethyl acetate. In GC-MS analysis the DB-5MS quartz capillary column with Electron Impact source(EI) were used for ketamin determination using SKF(525A )as a internal standard. Qualitative analysis can be condncted for morphine, Heroin and ketamin by the combined R(f) of drugs spots and the TLC spectrum scaning chart. RESULTSBy TLC method,ketamin, morphine and heroin had a good separation. In the GC-MS analysis, the linear range was (0.5)-(20.0) mg·L^( 1) (r=(0.998 7)). The detective limit was (0.2) mg·L^( 1) (S/N=3). CONCLUSIONThe TLC and GC-MS methods can be used to detect ketamin from drugs and for the forensic medical identification of ketamin abuse.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第6期497-499,共3页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金资助项目(编号:30371577)
山西医科大学学生创新项目基金资助项目(编号2004037)