氯胺酮的薄层层析和气相色谱质谱分析

Determination of ketamin by use of TLC and GC-MS

目的:建立氯胺酮的薄层层析法(TLC)和气相色谱质谱(GC-MS)测定方法。方法:样品在pH13条件下用醋酸乙酯萃取,SKF525A为内标,以DB5MS石英毛细管柱、EI源、不分流进样GCMS法测定氯胺酮药物;斑点颜色结合比移值和光密度扫描图TLC法定性分析吗啡、海洛因和氯胺酮。结果:TLC法中氯胺酮与吗啡、海洛因分离良好。GCMS分析中,氯胺酮药物血药浓度在0.5～20.0mg·L$C1范围内呈线性关系,r=0.9987,最低检出限为0.2mg·L$C1(S/N=3)。结论:建立的TLC和GCMS法可用于毒品中氯胺酮的检验和氯胺酮中毒的法医学鉴定。

OBJECTIVETo establish two methods for determining the ketamin by TLC and GC-MS. METHODSThe specimens were alkalized and extracted with ethyl acetate. In GC-MS analysis the DB-5MS quartz capillary column with Electron Impact source(EI) were used for ketamin determination using SKF(525A )as a internal standard. Qualitative analysis can be condncted for morphine, Heroin and ketamin by the combined R(f) of drugs spots and the TLC spectrum scaning chart. RESULTSBy TLC method,ketamin, morphine and heroin had a good separation. In the GC-MS analysis, the linear range was (0.5)-(20.0) mg·L^( 1) (r=(0.998 7)). The detective limit was (0.2) mg·L^( 1) (S/N=3). CONCLUSIONThe TLC and GC-MS methods can be used to detect ketamin from drugs and for the forensic medical identification of ketamin abuse.