高效液相色谱法测定疏肝舒乳颗粒中延胡索乙素的含量

HPLC determination of tetrahydropalmatine contained in Shuganshuru granules

目的:建立以高效液相色谱法测定疏肝舒乳颗粒中延胡索乙素含量的方法。方法:采用高效液相色谱法(HPLC)测定,色谱条件:色谱柱:HypersilOSD2C18(4.6mm×250mm,5μm);流动相:甲醇水(0.05mol·L-1磷酸氢二钠)(75∶25);检测波长:280nm;柱温:25℃;流速:0.9mL·min-1。结果:延胡索乙素在1.8～8.8mg·L-1范围内与相应的峰面积呈良好线性关系(r=0.9999)。加样回收率为98.5%。结论:本法具有操作方便、灵敏、稳定,与组方中其他药材成分无干扰,可作为该产品含量测定方法之一。

OBJECTIVETo establish a new method for the determination of tetrahydropalmatine in Shuganshuru granules by HPLC.METHODSHPLC was used. Chromatographic conditions: Hypersil OSD2 C_(18) ((4.6 mm)×(250 mm),( 5 μm)) column, methanol-water ((0.05 mol)[DK]·L^(-1) Na_(2)HPO_(4))(75[DK]∶25) as mobile phase, detecting wavelength was (280 nm), column temperature was (25 ℃), flow rate was (0.9 mL)[DK]·min^(-1 ).RESULTSThere was a good linear relationship in the range of (1.76)～(8.8 mg)[DK]·L^(-1)(r=(0.999 9)).The average recovery was (98.5%) with RSD of (2.3%).CONCLUSIONThe method is simple, accurate and reproducible.It is suitable for content determination of Shuganshuru granules.