蚯蚓-菌根在植物修复镉污染土壤中的作用

Roles of earthworm-mycorrhiza interactions on phytoremediation of Cd conta-minated soil

以灰化土(Aquods)为供试土壤,分别加入4个浓度的Cd2+(0,5,10,20mg/kg)模拟土壤污染,设置每钵接种8条蚯蚓(Pheretimasp.)、接种菌根(InoculumEndorize-Mix2)和同时接种蚯蚓和菌根的处理,以不加蚯蚓和菌根为对照,并种植黑麦草(Loliummultiflorum),研究蚯蚓菌根相互作用对Cd污染土壤中黑麦草生长及土壤中Cd生物有效性的影响。结果表明菌根浸染率不受添加Cd浓度的影响,平均浸染率为22%,加入蚯蚓能使菌根的侵染率提高9%。在Cd污染土壤上,引进蚯蚓显著增加了黑麦草地上部的生物量,接种菌根对黑麦草地上部分产量没有明显影响,同时接种蚯蚓和菌根与只接种蚯蚓相比没有显著差异。蚯蚓活动显著提高了土壤中CaCl2-Cd的含量,而菌根只在低浓度Cd处理上增加了土壤中CaCl2-Cd含量,二者对H2O-Cd、DTPA-Cd均无显著影响,蚯蚓和菌根对增加土壤有效态Cd含量不存在协同作用。蚯蚓活动促进了黑麦草对Cd的吸收,但吸收的Cd积累于黑麦草根部。接种菌根不仅能促进黑麦草对Cd的吸收,而且还能促进Cd从植物的根部向地上部分转移,由于接种蚯蚓可以提高菌根的浸染率,所以二者具有促进Cd向地上部转移的协同作用。这对于重金属污染土壤的植物修复具有十分重要的意义。

There has been increasing interest in developing a plant-based technology (phytoremediation) to remediate heavy metal-contaminated soils. The primary objective of this technology is to maximize the transfer of heavy metals to plants so that the greatest total mass of contaminant is removed by each cropping. Slow growth rate and low biomass of hyperaccumulating plants may limit the utility of phytoremediation technology. In addition, the low bioavailability of heavy metals in the soil also restrains this technology application. Earthworm is an important components of plant rhizosphere ecosystem, and it significantly contributes to total soil organic matter, enhance nutrient cycling, improve soil physical conditions, modify soil pH and promote plant growth, and able to increase metal bioavailability in soil through burrowing and casting. The arbuscular mycorrhiza (AM) fungi are important rhizospheric microorganisms. They can increase plant uptake of nutrients and consequently increase root and shoot biomass and improve plant growth. Available evidences suggest that AM fungi can colonize plant roots in metal contaminated soil, while their effects on metal uptake by plant are conflicting in previous studies. In order to understand thecomplex interactions between roots, earthworms and arbuscular mycorrhiza (AM) in the rhizosphere in metal contamination soil, present study focuses on investigating the effects of inoculation of earthworms and/or arbuscular mycorrhiza (AM) on ryegrass growth and bioavailability of Cd in Cd-contaminated soil. Aquods from Laitiao Village, Hong Kong was used in the laboratory incubation experiment. The soil had a pH (in water) of 6.73, and the concentration of DTPA extractable Cd (at pH 7.3) was undetectable. The soil was steam-sterilized (121℃ for 2 h) by autoclaving to eliminate native AM propagules. Soil in pots (1.0 kg per pot) was amended to contain 0, 5, 10 and 20 mg Cd/kg by adding CdCl_2. After incubation for 2 months at 20℃ and moisture content of 70%, all the pots were divided into four groups, with one following treatment respectively: earthworm [8 individual earthworms (Pheretima sp.) per pot], arbuscular mycorrhiza (AM)[30 g/kg soil], combination of earthworms and AM, and without earthworm and AM. Each pot was received 15 pre-germinated ryegrass (Lolium multiflorum) seeds. The earthworms used for the present experiment were washed free of surface soil with distilled water and kept in sterilized glass vessels for 24 h to minimize the number of naturally-occurring mycorrhizal propagules associatied with their surfaces or gut contents. Eight earthworms with similar fresh weight (0.6 g). The arbuscular-mycorrhizal fungi (AMF) inoculum was a mixture of Glomus mosseae and Glomus intraradices (Inoculum Endorize-Mix2) purchased from Biorize Sarl, France. Seeds of ryegrass (L. multiflorum) were surface sterilized in a 10% (v/v) solution of hydrogen peroxide for 10 min. The results from this study showed that that both earthworms and mycorrhiza were able to survive in all the Cd treatments after five weeks, but the growth of earthworms declined with the increase of Cd added. Compared with non-earthworm, earthworm treatment increased 9% root infection rate, significantly increased shoot biomass of ryegrass, increased Cd concentration in soil extracted by 0.01 mol/L CaCl_2 in all Cd treatments, and resulted in the increase of root Cd concentration. Compared with non-inoculation, inoculation mycorrhiza alone did not affect ryegrass biomass, however, significantly increased Cd concentration in shoot and root. Earthworms-mycorrhiza combination decreased shoot biomass of ryegrass compared with earthworms alone, and increased shoot Cd concentration in the treatments of 5 and 10 mg Cd/kg soil, when compared with earthworms or mycorrhiza alone. In conclusion, earthworms, mycorrhiza and their combination may have potential roles in increasing plant biomass and enhancing metal uptake by plant, and consequently elevating phytoextraction efficiency in low to medium level metal contaminated soil. Further in