偏侧帕金森病猴模型的脑血流灌注SPECT活体显象研究

In Vivo Tomographic Study of Cerebral Blood Perfusion with SECT in Hemiparkinsonian Monkeys

给恒河猴右侧颈内动脉注射神经毒药物甲基-苯基-四氢吡啶(MPTP)后,产生右侧黑质神经元脱失,黑质纹状体多巴胺(DA)浓度明显降低,以及左侧帕金森样症状。投予抗帕金森病(PD)药物治疗显著地改善帕金森样症状。采用单光子发射计算机断层扫描(SPECT),以^(99m)Tc-ECD作为显象剂显示偏侧PD猴模型的脑血流灌注,发现猴模型建立3个月内的损毁侧脑的脑血流灌注明显减低,8个月时的损毁侧脑的脑血流灌注转为正常。结果表明脑血流灌注的变化反映了PD的病理生理变化过程。

We present data on the utility of functional brain imaging with 99mTc-ECD and SPECT in the study of MPTP-induced hemiparkinsonism in the monkeys. Administration of MPTP into the right common carotid artery of 10 rhesus monkeys produced hemiparkinsonism in the contralateral limbs which responded to antiparkinsonian medication. The unilateral neurotox-icity of the MPTP-treated side was confirmed biochemically by marked reduction of contents of DA in the nigrostriatum and histologically by selective neuronal loss in the substantia nigra. These monkeys with hemiparkinsonism were studied by using SPECT and 99mTc-ECD as perfusion marker. The results of brain scanning showed that the cerebral blood perfusion of MPTP-treated side was significantly depleted 20-90 d after MPTP intoxication, and re- turned to the normal state of perfusion 8 months following it. The experiment indicates that the abnormalities of cerebral blood perfusion are involved in the course of parkinsonian pathophysiology.