癫痫易感大鼠脑内CCK-8肽含量、CCK基因结构及CCK-mRNA表达初探

A Preliminary Research about the CCK-Octapeptide Content, CCK-mRNA Expression and CCK Gene Structure in Developmental Epilepsy-Prone Rat Brain

对听源性癫痫易感大鼠P_(77)PMC及对照Wistar大鼠发育中脑内基础CCK-8肽含量、mRNA表达水平及胆囊收缩素(cholecystokinin,CCK)基因结构进行初探。提示:(1)P_(77)PMC在发育中,成年脑内基础CCK-8肽显著高于对照;(2)Northern印迹杂交示P_(77)PMC组脑CCK-mRNA与对照无显著差异;(3)CCK DNA结构在两组大鼠未发现RFLPs不同。证实两系大鼠脑内CCK系统存在差异,其与癫痫易感性的关系尚待进一步研究。

By utilyzing audiogenic epilepsy-prone rat P77 PMC matched with epilepsy resistant rat Wis-tar, the CCK-mRNA expression in whole brain and brain CCK gene structure of both rat groups were studied. The results showed; (1) Total brain CCK-8 peptide content is significantly higher (F<0. 05) in P77 PMC rat brain than that of Wistar rat during development, but the increasing extent of CCK-8 with increasing age is lower in P77 pMC rat. (2) Total brain CCK-mRNA expression is no significant difference between P77 PMC and Wistar rats. (3) There are no differences in CCK DNA structure and RFLPs between P77 PMC and Wistar rats brain. Our results have shown that there is indeed some difference in CCK system between two groups, but it is not clear whether the increased total brain is due to the compensation for the CCK receptor functional deficiency or the regulation of CCK gene post-translational modification by other factors. The exact relationship between our results and the epilepsy susceptibility of P77 PMC rat need further studies.