酪氨酸羟化酶cDNA移植治疗帕金森病的实验研究

Treatment of Parkinson's Disease by Transplantation of Tyrosine Hydroxylase cDNA: An Experimental Research

帕金森病(PD)的主要病因是黑质多巴胺能神经元损伤,表达的酪氨酸羟化酶(TH)减少。本文在应用6-羟多巴胺(6-OHDA)制备偏侧PD大鼠模型的前提下,首次将TH cDNA移植到PD大鼠纹状体内,模型动物的旋转行为明显改善,用免疫组化法及PCR法证实了外源性THcDNA可以进入脑细胞内,并表达出有生物活性的TH。

Parkinson's disease is a neurodegeneration disorder characterized principally by a loss of dopaminergic cells in the substantia nigra, and reduction of gene expression of tyrosine hydroxylase. A rat model of Parkinson's disease was induced by unilateral administration of 6-OHDA into right substantia nigra. Tyrosine hydroxylase cDNA was grafted into the lesioned striatum of the rat model of Parkinson's disease to compensate the dopaminergic deficit. The results indicate that a partial reversal of rotational behaviour of the rat model was obtained. By immunohis-tochemical and PCR methods it was observed that exogenous tyrosine hydroxylase cDNA directly injected into striatum could be incorporated with neurons , and it could express bioactivity in the striatum.