联合检测血清酯酶和乳酸脱氢酶同功酶对原发性肝癌诊断价值的初步探讨

THE STUDY ON THE DIAGNOSTIC VALUE OF MEASUREMENT OF ES AND LDH ISOENZYME IN PRIMARY LIVER CANCER

目的:研究使用同功酶临床诊断试剂盒(醋酸纤维素薄膜电泳法)联合检测血清酯酶(Es)同功酶和乳酸脱氢酶(LDH)同功酶对肝硬化和肝细胞癌(HCC)的诊断价值。方法:采用Es试剂盒和LDH试剂盒检测血清,原发性肝癌病人18例,失代偿期肝硬化病人40例,正常人40例。结果:HCC及肝硬化病人Es2带活性减弱甚至大多数消失,与正常对照相比差异有极显著性(P<0.01),而HCC病人LDH5带活性明显增强,与肝硬化及正常相比差异有显著性(P<0.05)。结论:检测血清Es对肝硬化和HCC的诊断有高度敏感性,结合检测血清LDH对肝硬化并发HCC的诊断具有重要的临床价值。

Objective: To analyse diagnostic value of Es and LDH isoenzyme measurement in cirrhosis of liver and primary hepatocellular cance r(HCC). Methods: Serum esterase(Es) isoenzyme and lactate dehydrogen ase(LDH) isoenzyme were measured by using clinic isoenzyme diagnosis Kit on 18 cases with primary HCC, 40 cases with cirrhosis of liver and 40 cases of normal controls. Results: The study suggested that the activity of Es2 in cases with HCC and cirrhosis of liver was weakened or even disappeared in most of them, and it was significantly different when compared with that of controls (P<0.01). The activity of LDH5 was strengthened in cases with HCC and it w as significantly different when compared with that of the cases with cirrohosis of liver and control (P<0.05). Conclusion: Measurement of Es ha d higher sensitivity to diagnosis of cirrhosis of liver and HCC and it had an im portant clinical value if used together with measurement of LDH to diagnosis of cirrhosis of liver complicated by HCC.;