鱼腥藻7120质膜、类囊体膜和细胞壁的分离及特性分析

ISOLATION AND CHARACTERIZATION OF THE CYTOPLASMIC, THYLAKOID MEMBRANES AND CELL WALLS OF ANABAENA 7120

于1987－1989年对鱼腥藻7120质膜、类囊体膜和细胞壁进行分离纯化和基本的色素与蛋白质特性分析。采用机械性方法破碎细胞，以非连续蔗糖密度梯度离心分离纯化鱼腥藻7120营养细胞的质膜和类囊体膜，以TritonX－100处理方法获得细胞壁。色谱分析和电泳结果表明，其质膜、类囊体膜的色素和蛋白质特性与单细胞蓝藻相类似；Triton不溶细胞壁未发现可见光谱吸收，其两条主要蛋白带52KD和14KD均为精蛋白，并且14KD蛋白为肽聚糖层连结蛋白，细胞壁蛋白质全部对胰蛋白酶处理敏感，其上未发现“热修饰”蛋白。

Isolation of the cytoplasmic and thylakoid membranes, and cell walls of Anabaena 7120 and analysis of the features of their pigments and proteins were studied during 1987-1989. The cytoplasmic and thylakoid membranes of Anabacna 7120 were isolated by mechanical disruption of the vegetative cells followed by discontinuous sucrose density gradient centrifugation. The cell walls of Anabaena 7120 were obtained by the Triton X-100 treatment method. The features of pigments and proteins of cytoplasmic and thylakoid membranes of Anabaena 7120 were similar to those of unicellular blue-green algae. The Triton-insoluble cell wall of Anabaena 7120 had no spectral absorption in visible light. Its SDS-PAGE map showed two major polypeptide bands with apparent MW. of 52KD and 14KD and both of them were glycoproteins. 14KD was peptidoglycan-associated protein. Almost all of the cell wall proteins were sensitive to trypsin treatment. The 'heat-modifiable protein' was not found in the cell walls of Anabaena 7120.