基因重组乳链球菌防龋疫苗的研制　Ⅱ．变形链球菌结构基因重组质粒对乳链球菌的克隆

Study of Oral Vaccine Against Dental Caries of Gene Recombination in Streptococcus Lactis Ⅱ.Cloning of Streptococcus Lactis by Recombinant Plasmid of Streptococcus Mutans Structural Gene

在获得带有结构基因的质粒ｐＰＣ４１和穿梭质粒ｐＳＡ３的基础上，制备目的基因并与载体ＤＮＡ进行体外连接，使重组体导入乳链球菌ＬＭ０２３０受体细胞，为研制防龋疫苗获得携带变链菌表面抗原基因的重组乳链球菌。

Abstract Bases on the plasmids pPC41 containing the structural gene(pac)and E.coli-S.lactis shuttle vector pSA3.were obtained.The fragment containing the pac gene was isolated and it was ligated to vector.The reconbinant was used to transform S.lactis LM0230 cells.The recombinant S.lactis strains HL45,HL102 and HL107 et al.which carries the structural gene for a surface protein antigen (PAc) from S.mutans was constructed for development of an oral vaccine against dental caries.