牙源性肿瘤中NEU基因产物表达分析

Overexpression of Neu Gene in Odontogenic Tumors

ｎｅｕ基因（又叫做Ｃ－ｅｒｂＢ－２和ＨＥＲ－２基因），其表达产物为１５０～１９０ｋＤ的糖蛋白，与表皮生长因子受体在结构上十分相似。本研究利用ｎｅｕ基因表达产物的单克隆抗体观察了ｎｅｕ基因蛋白产物在牙源性肿瘤中的分布，借以探索牙源性肿瘤的发生与ｎｅｕ基因扩增的关系。结果发现，正常人牙胚的造釉细胞和造牙本质细胞呈弱阳性；２２例造釉细胞瘤中１６例阳性；６例牙源性腺样瘤中５例阳性；３例牙源性钙化上皮瘤均为阳性。因此，牙源性肿瘤的发生可能与ｎｅｕ基因的过量表达有关。

Neu gene(C-erb B-2 or HER-2 gene) was a kind of proto-oncogene, its expression product was 150-190 kD protein,and its structure was similar to that of epithelial growth factor receptor. In this study, the expression of neu gene in odontogenic tumors, including ameloblastomas (22 cases), odontogenic adenomatoid tumor (OAT, 6 cases), and calcifying epithelial odontogenic tumor (CEOT,3 cases), was observed using immunohistochemistry in order to study the relationship between the development of odontogenic tumors and the expression of neu gene. The results showed that, of 22 cases of ameloblastomas, 16 were positive; of 6 cases of OAT, 5 positive; all 3 cases of CEAT ware positive for neu. So the development of odontogenic tumors might be associated with the overexpression of the neu gene.