牙周病龈组织中血栓素B_2的放射免疫测定及免疫组化定位与定量分析

The Location and Quantitative Analysis of TXB_2 in Gingiva of Patients with Periodontal Diseases by Radioimmunoassay and Immunohistochemistry

选取成人牙周炎、青少年牙周炎、龈炎和健康人各１０例，用放射免疫测定法测量龈组织中血栓素Ｂ２（ＴＸＢ２）含量，用免疫组化ＡＢＣ染色对同一标本中ＴＸＢ２定位，用显微分光光度计（ＭＳＰ）对染色标本定量。结果表明：牙周病患者龈组织中ＴＸＢ２含量从健康人龈炎、青少年牙周炎到成人牙周炎呈递增趋势，且各组间均有显著性差异。ＴＸＢ２主要的分泌细胞为血管内皮细胞、上皮基底细胞、巨噬细胞和浆细胞，对牙龈组织中ＴＸＢ２的ＭＳＰ定量结果与放射免疫测定结果相似，提示进一步开展ＴＸＢ２与牙周病之间关系的研究有重要意义。

Abstract This investigation selected the patients with adult periodontitis(AP),juvenil periodontitis(JP), gingivitis(G)and health(H)to analyse the contents of thromboxane B2(TXB2) in gingiva by radioimmunoassay(RIA),to locate the TXB2 of the same sample by immunohistochemistry avidin-biotin-complex(ABC),and quantitative analyse the contents of TXB2 in same sample by microspectrophotometer(MSP).The results had shown that the contents of TXB2 in gingiva were increased from H,G,JP to AP. There was a significant difference between each group. The main secretory cells of TXB2 were vascular endothelial cells, basel cells, macrophages and plasmacytes.The measurements of the TXB2 contents in gingiva with periodontal disease by RIA are in accordance with those obtained by MSP,which suggests the importance of studying the relationship between TXB2 and periodontal disease.