嗜盐隐杆藻胞外多糖抗肿瘤活性研究

Antitumor activity of exopolysaccharide from Aphanothece halophytica

目的探讨嗜盐隐杆藻胞外多糖(exopo lysaccharide of Aphanothece ha lophy tica,EPAH)的抗肿瘤活性。方法以S180肉瘤为肿瘤模型,检测EPAH对肿瘤生长的抑制活性;M TT法检测EPAH对S180肉瘤细胞、Sm cc7721肝癌细胞和H eL a细胞的体外抑制活性;以对荷瘤小鼠免疫器官、血液淋巴细胞数量及淋巴细胞增殖影响、NK细胞杀伤活性和巨噬细胞产生NO及其他细胞因子的影响评价EPAH对小鼠免疫功能的作用。结果EPAH对S180肉瘤生长有显著的抑制作用,其中预防给药和与肿瘤接种同时给药组最大抑瘤率分别达66.79%和47.93%。100μg/mL EPAH对Sm cc7721细胞、H eL a细胞以及S180细胞生长的抑制率达60%以上。EPAH显著提高荷瘤小鼠脾脏和胸腺质量以及血液淋巴细胞数量,促进淋巴细胞增殖反应,增加NK细胞杀伤活性,刺激巨噬细胞产生NO,分泌白细胞介素-1β(IL-1β)及肿瘤坏死因子-α(TNF-α)。结论EPAH有显著的抗肿瘤活性,并能直接作用于肿瘤细胞,抑制肿瘤生长。EPAH可能是通过增加荷瘤小鼠免疫器官的质量和免疫细胞的数量、促进淋巴细胞分裂,提高NK细胞杀伤活性和巨噬细胞产生NO以及其他细胞因子实现其抗肿瘤活性。

Objective To elucidate the antitumor activity of exopolysaccharide from Aphanothece halophytica(EPAH).Methods The in vivo inhibition of EPAH on growth of tumor was performed by inoculation of S_(180) sarcoma cells into ICR mice.While in vitro activity against tumor cells was assayed by the growth inhibition of cell lines of S_(180) sarcoma,Smcc7721,and HeLa.The effects of EPAH on immune function were evaluated by the influence on the thymus,spleen,and the number of lymphocytes in blood stream,the influence on proliferation of lymphocytes,the killing activity of NK cells,and the production of NO,IL-1β,and TNF-α. Results EPAH inhibited in vivo S_(180) sarcoma growth with the highest inhibi-(tory) rate of 66.79% and 47.93% in the test mice of pretreatment and simultaneous treatment,respectively.EPAH also displayed in vitro activity against the test cell lines with the highest inhibitory rate being more than 60% at a concentration of 100 μg/mL.EPAH was found to affect the immune function in mice including increasing the weight of thymus,spleen,and the number of lymphocytes in the blood stream,accelerating the proliferation of lymphocytes,enhancing the killing activity of NK cells,and stimulating the production of NO,IL-1β,and TNFα by macrophages.Conclusion EPAH is an effective antitumor(agent.) It inhibites the tumor cells directly and hence the growth of tumor.Its antitumor activity is probably realized by increasing the weight of immune organs and the number of immunocytes as well as lymphocyte proliferation,enhancing the killing activity of NK cells,facilitating the production of NO and related(cytokines) in tumor-bearing mice.