小鼠及人正常组织谷胱甘肽－S－转移酶π基因与三种癌基因的分布和相互关系的研究

DISTRIBUTION AND RELATIONSHIP BETWEEN GLUTATHIONE S-TRANSFERASEπ(GSTπ)GENE AND-myc,v-erb-B AND N-ras ONCOGENS IN MICE AND HUMAN NORMAL TISSUE

用Ｈａｂｉｇ改良法测定人及ＤＡＢ小鼠正常组织中谷胱甘肽－Ｓ－转移酶活性及π基因与３种癌基因的分布。人７种正常组织中ＧＳＴπＲＮＡ相对含量为４．８６～０．２５，以胎盘最高，其次为膀胱、肺、胃。小鼠的ＧＳＴ活性为１７．７～１２．４７，肝最高，其次为胃、肺和肾。ＧＳＴπＲＮＡ为１．９８～０．４２５，以胃最高，脾和肺次之，ＧＳＴ活性与ＧＳＴπＲＮＡ的检测结果基本一致。人７种正常组织中，Ｎ－ｒａｓ的相对含量为２０～２．９４，以胎盘含量最高，直肠、乳腺次之。Ｃ－ｍｙｃ为２３．４～７．３８，胎盘、肺、膀胱较高。ｖ－ｅｒｂ－Ｂ为９．０５～０．３８，以胎盘最高，直肠、胃次之。在小鼠６种组织中，Ｎ－ｒａｓ的基因相对含量为６．２３～１．０４，以肺、脾较高，胃、肝、心、肾次之。Ｃ－ｍｙｃ为１３．７６～５．３１，肺、胃较高。ｖ－ｅｒｂ－Ｂ为８．７２～２．０９，肺、胃较高，脾次之。说明ＧＳＴπ对肿瘤的发生有一定的制约作用。

We studied the distribution and the relationship between GSTπ gene and C-myc,v-erb-B,N-ras oncogenes in mice and human normal tissues.The hybridization relative value of GSTπ RNA was about 4.86-0.25 in 7 kinds of human normal tissues and the highest in placenta,then bladder,lung,gastric tissues and the same as the GST activity.The GST activity is about 17.7-12.47 in 6 kinds of mice nornal tissues and the highest in liver,then gastric,lung,kindey,The GSTπ RNA is about 1.98-0.425 and the highest in placenta,next spleen,lung.The hybridization relative value were about 20-2.94 that the highest in placenta,next to rectal,breast for N-ras and 23.4-7.38 that higher in placenta,lung,bladder for C-myc and 9.05-0.38 that the highest in placenta,then rectal,gastric for v-erb-B in 7kinds of human normal tissues.The hybridization relative value were about 6.23-1.04 that higher in lung,spleen,then gastric,liver,heart, kindey for N-ras and 13.76-5.31 that higher in lung, gastric for C-myc and 8.72-2.09 that higher in lung, gastric,then spleen for v-erb -B. These results suggested that there would be a relationship between the GST activity or GSTπ RNA and the oncogene.The GST or GSTπ as the factors of inhibit tumor could inhibit the amplification of oncogene.