人鼻咽癌CNE－2Z细胞的不同转移潜能克隆株在严重联合免疫缺陷小鼠体内的检验和比较研究

VERIFICATION AND COMPATIVE STUDY OF HUMAN NASOPHARYNGEAL CARCINOMA CNE-2Z VARIANT CELLS WITH DIFFERENT METASTATIC POTENTIAL IN SEVERE COMBINED IMMUNODEFICIENCY(SCID)MICE

将人类鼻咽癌不同克隆株的细胞悬液移植在严重联合免疫缺陷（ＳＣＩＤ）小鼠和ＢＡＬＢ／ｃ（ｕｎ／ｕｎ）裸小鼠的颈背侧皮下，５６ｄ后处死全部动物进行观察。结果发现，在ＳＣＩＤ小鼠体内移植后ＣＮＥ２Ｌ２为高转移克隆株，其淋巴结转移率为１００％，肺转移率为７１％；而ＣＮＥ２Ｌ４为低转移克隆株，其肺转移率为１３％，淋巴结未见癌转移。这是从１个细胞母系中新筛选出的１个高转移和１个低转移的癌细胞克隆株。实验结果还显示，同ＢＡＬＢ／ｃ（ｕｎ／ｕｎ）裸小鼠相比，ＳＣＩＤ小鼠的恶性表型的表达能力高。另外，皮下移植时肿瘤细胞的数量可能与转移表型的表达有相关性，移植的瘤细胞数越多，转移率越高，反之亦然。

Cloned CNE-2Z variants cells(CNE-2L2 and CNE2L4)in suspension form were injected into subcutaneous costal region of SCID mice(severe combined immunodeficiency mice)and BALB/c(nu/nu)nude mice.All tumor bearing mice were sacrified on 56th day after transplantation.The results indicate that CNE2L2 tranplanted into SCID mice is a highly metastatic variant which produced lymphatic metastasis in 100%(7/7)of the cases and lung metastasis in 71%(5/7)of the cases, while CNE2L4 transplanted into SCID mice is a low metastatic variant which produced lung metastasis in 13%(1/8)of the cases and no lymphatic metastasis is found.The results also indicate that SCID mice can benefit the expression of metastasis phenotype of the transplanted cells,and suggest that SCID mice can be a more suitable in vivo model compared to BALB/c(nu/nu)nude mice for study the cancer biological characters.This experiment also shows that the tumor cell number inoculated into subcutaneous costal region are related to the expression of metastasis phenotype higher rate of metastasis result from more numbers of cells transplanted subcutaneously.