醋酸棉酚和高能震波对人膀胱癌细胞系单独和联合作用的研究

STUDY ON THE EFFECTS OF ACETATE GOSSYPOL,HIGH ENERGY SHOCK WAVES(HESW)AND THEIR COMBINATION ON THE HUMAN BLADDER CANCER CELL LINE BT5637

膀胱癌细胞系ＢＴ５６３７经醋酸棉酚或高能震波单独作用和两者联合处理后，癌细胞生长增殖受抑制，分裂指数及集落形成率下降。３Ｈ－ＴｄＲ掺入减少。Ｆｅｕｌｇｅｎ染色后经显微分光光度计检测显示ＤＮＡ含量降低。流式细胞计分析发现醋酸棉酚组Ｇ０／Ｇ１期比例显著增高，Ｓ、Ｇ２／Ｍ期比例减少；震波组Ｓ期比例下降。本研究提示，醋酸棉酚可阻断癌细胞由Ｇ０／Ｇ１期进入Ｓ期，而高能震波则抑制Ｓ期。电镜观察显示，醋酸棉酚及高能震波对超微结构的影响是多方面的，主要是线粒体的改变。以上各项指标的改变在联合组均较单独处理组更显著。生长曲线分析还揭示，醋酸棉酚对癌细胞杀伤和抑制作用与药物剂量和作用时间有关，而且是可逆的，高能震波冲击后细胞生长暂时受抑制。Ｎｏｒｔｈｅｒｎ印迹杂交分析显示醋酸棉酚可抑制Ｃ－ｍｙｃ基因表达。

The growth potential of human bladder cancer cell line BT5637 was inhibited by acetate gossypol or high energe shock wave(HESW)and more greatly their combination as shown by cell growth curve,mitosis index and colony-forming rate.The growth curve showed that the effects of gossypol were reversible and related to the duration of action and the concentration of the drug.Exposure to HESW resulted in temporal growth delay.3H-TdR incorporation test and measurement of DNA content by microspectrophotometer showed that gossypol or HESW and their combination exerted their action on DNA synthesis.Treatment with gossypol and HESW or both also resulted in the percentage change of the cell numbers in G0/G1,S or M phases as determined by flow cytometer.It suggested that gossypol could block cells from G0/G1 phase to S phase,and HESW perhaps inhibited S phase,Electronmicroscopic study showed that the ultrastructural changes produced by gossypol,HESW or both manifested themselves in several aspects.The prominent changes were the swelling of mitochondria as well as vesiculation of endoplasmic reticulum.Northern blot result indicated that the expression of C-myc gene was inhibited by acetate gossypol and the growth of BT5637 cells was probably associated with C-myc gene expression.Taking all the data mentioned above, our experiment demonstrated that acetate gossypol or HESW could inhibit the cell growth, while the combination of the two agents might have synergistic effect on the bladder cancer cells.