摘要
用Ｓｅｐｈａｄｅｘ Ｇ－５凝胶过滤层析法，进一步纯化具肝抑素生物活性的大鼠肝蛋白质粗提品。以分离的大鼠再生肝的肝细胞为靶细胞，体外检测各洗脱峰浓缩物对肝细胞增殖的抑制率。结果证明，Ｅ峰浓缩物的抑制作用最强，其活性比为粗提品的２０倍。ＳＤＳ聚丙烯酸胺电泳图及蛋白质迁移率测定表明，该浓缩物的主要成分为分子量１３．５ｋＤ的多肽。本研究对大鼠肝抑素做了初步纯化，验证了该物质在肝再生中起重要调控作用的生物效应。
The hepatic chalone was partially purified from rat liver extract(Fraction Ⅱ)by Sephadex G-50 gel.chromatography in this study,The cultured hepatic cells isolated from regenerating rat livers after subtotal hepatectomy were used as the target cells,and inhibiting effects of FractionⅡ and each wave concentrate of Sephadex G-50 gel chromatography on proliferation of cultured hepatic cells were assayed in vitro.The results indicate that the concentrate of wave E exhibit the most powerful inhibiting effect which is 20 times stronger than that of Fraction Ⅱ.SDS-PAGE and migration rate show that the main component of this concentrate is a polypeptide with a molecular weight of 13.5 kD which may exert an important regulating effect on liver regeneration.
出处
《解剖学报》
CAS
CSCD
北大核心
1994年第3期308-312,T015,共6页
Acta Anatomica Sinica
基金
国家教委博士点专项资金
关键词
肝再生
肝抑素
凝胶层析
Liver regeneration
Hepatic chalone
Sephadex G-50 gel chromatography
Subtotal hepatectomy
Rat
