正常胃粘膜、癌周粘膜和癌组织中Cu Zn SOD免疫组化研究

The Immunohistochemical Study of Cu Zn SOD in Normal Gastric Mucosa and Peripheral Tissue Near Cancer and Cancer Tissue

本文首次报道了利用兔抗人Cu Zn SOD抗血清对正常胃粘膜和癌周及癌组织中Cu Zn SOD分布,进行免疫组化定位研究。发现正常胃粘膜组织中Cu Zn SOD染色强度明显高于癌周粘膜(P<0.05);癌周粘膜组织明显高于癌组织(P<0.05)。表明胃癌细胞中Cu Zn SOD具有明显降低的现象。结果还显示了39例胃癌组织中Cu Zn SOD染色阳性强度与术中所见腹腔转移有一定的关系,表现为中度阳性组其腹腔转移率(37.5％)低于弱阳性和阴性组(51.3％)。本文还对上述结果形成机制和外源性SOD制剂的临床应用做了简要探讨。

Immunohistochemical localization of Cu Zn SOD in normal gastric mucosa, peripheral tissue near cancer and cancer tissue was carried out by using rabbit anti-human Cu Zn SOD serum. The results showed that Cu Zn SOD stainning in normal mucosa was stronger significantly than in peripheral tissue (P<0. 05), and the latter stronger than that in cancer tissue (P <0. 05). These demonstrated that Cu Zn SOD was decreased in gastric cancer cell and that CuZn SOD stainning degree in 39 cases of cancer was related with abdominal metastasis. The metastatic percentage in mid and strong postive groups (37. 5%) was lower than that in weak postive groups and negative groups (68.9%). The formative principles of above results and the clinical use of foreign SOD phamaceutics were discussed briefly.