类细菌素高产菌YCF10的诱变选育及发酵条件的研究

Research on Mutation and Screening and Fermentation Conditions of YCF 10 Strain for High Productivity of Bacterioncin-like Substance

以布氏乳杆菌(Lactobacillusbuchneri)CF10为出发菌株,经紫外线照射(UV)和硫酸二乙酯(DES)多轮复合诱变,获得一株高产类细菌素的目的菌YCF10,发酵液相对效价较未诱变前提高了136%,且具有良好的传代稳定性。通过对该菌株产类细菌素发酵条件的研究发现,以2%麦芽糖为碳源、1%大豆蛋白胨加0.5%酵母粉为氮源,加微量K+,Mg2+和Mn2+等金属离子,培养温度37℃,初始pH6.0～6.5,接种量1%,厌氧条件下,培养时间48h,YCF10所产类细菌素的抑菌活性最高。

Lactobacillus buchneri CF 10 was used as start strain to screen a strain YCF 10 with high productivity of bacterioncin-like substance by UV radiation and multiple times compounding mutation of diethyl sulphate(DES).The relative titer of fermenting liquid increased by 136 % after mutation and the liquid possessed good passage stability.And the following fermentation conditions were the best for YCF to produce bacterioncin-like substance of the highest activity:2 % maltose as carbon source,1 % soybean peptone and 0.5 % yeast powder as nitrogen source, addition of minute quantities of metal irons such as K+,Mg2+ and Mn2+,culture temperature at 37 ℃,initial pH value as 6.0～6.5,1 % inoculation quantity,and 48 h culture time under anaerobic conditions.