摘要
考察了微量元素对乙酰乳酸脱羧酶活的影响,通过单因子实验确定微量元素的最佳产酶浓度,进一步通过回归方法计算出微量元素的理论最佳产酶浓度,并用响应面分析法确定微量元素的最佳配比:微量元素配比为Mg2+0.022g/L,Mn2+0.00076g/L,Zn2+0.00296g/L,酶活提高了3倍。
The effects of trace elements on ALDC activity were investigated and the optimal trace elements concentration was confirmed through single factor experiments. Furthermore, the optimal theoretical trace elements concentration was calculated through retrogressive method. And the optimal proportioning of trace elements was verified as follows by response surface method: Mg2+0.022 g/L,Mn2+0.00076 g/L and Zn2+0.00296 g/L, which could improve enzyme activity three times of the original value.
出处
《酿酒科技》
北大核心
2005年第7期81-83,共3页
Liquor-Making Science & Technology
基金
河北省重点攻关课题
课题编号:94245509D
关键词
乙酰乳酸脱羧酶
微量元素
响应面
acetolactate decarboxylase (ALDC)
trace elements
response surface
