用微卫星标记定位太空诱变玉米核不育基因

Locating Maize Male Sterility Gene Induced by Space Flight Using Microsatellite Markers

用姊妹交多代的太空诱变玉米雄性不育材料RP3195(A)×S37(自交系)的两个不同果穗的F2代群体作为育性调查和基因定位群体,这两个果穗的F2代群体分别为138株和247株。用326对微卫星引物进行差异筛选,其中有56对引物出现多态性,然后用56对引物对F2代群体进行分析,结果表明引物bnlg197和umc1012与不育基因连锁,其中在F2代群体的不同果穗中引物bnlg197与不育基因之间的遗传距离分别为7cM和14.5cM,标记umc1012在F2代群体(138株)中与不育基因之间的遗传距离为28.5cM,据此将该核不育基因定位在3L染色体上。

Two F_2 populations with different kinds of spike derived from maize male sterility materials RP^3195(A)× S 37 (inbred line),which had been sib-bred for many generations,were used for sterility analysis and gene location.There were 138 and 247 plants in the two F2 populations respectively.Among the 326 pairs of microsatellite primers selected,56 were found polymorphic.Linkage analysis of F2 populations with the 56 pairs of primers showed that microsatellite markers bnlg197 and umc1012 were linked with the male sterility gene.The genetic distance between marker bnlg197 and the male sterility gene in the two different F2 populations were 7 cM and 14.5 cM respectively.The genetic distance between marker umc1012 and the male sterility gene in the 138 plants was 28.5 cM.Thus the male sterility gene was located on chromosome 3L.