大鼠脊髓横断损伤后移植胚胎5－羟色胺能神经元的研究

STUDIES ON TRANSPLANTATION OF FOETAL SEROTONERGIC NEURONS INTO THE TRANSECTED SPINAL CORD OF ADULT RATS

成年ＳＤ大鼠于第九胸髓（Ｔ９）处横断损伤，一周后将大鼠胚胎脑干中缝组织细胞悬液植入Ｔ９以下的脊髓内，动物存活１～１２月，分期处死取材，作５－羟色胺（５－ＨＴ）免疫组化的光镜和电镜观察。结果：脊髓损伤后在损伤部位尾侧的脊髓内５－ＨＴ阳性标记的纤维末梢消失，移植后于植入部位见许多５－ＨＴ阳性标记细胞，其突起在脊髓灰质内长距离延伸，末梢的分布与正常动物相似，并在免疫电镜下见到５－ＨＴ能神经末梢的突触形成。

Adult male SD rats underwent a complete transection of the spinal cord at the lower thoracic T,level.One week later a cell suspension,prepared from the raphe region of rat embryos,was transplanted into the spinal cord of rat below the level of the transection.The rats were sacrified after survival periods of 1 month to 1 year.The relevant processed spinal cord sections were treated for 5-HT immunochemical detection under light and electron mieroseopy.Immunoreactive 5-HT perikarya were located within the grafted regions of the rats.Axons of the grafted 5-HT cells grew and extended into the gray matter of the host,in a manner similar to the intaet animal.Established synapses showed in the reinnervated areasi whereas,in the control non-transplanted animals,there was a total absence of 5-HT immunoreactivity below the transection.