期刊文献+

81例女性淋病患者孔蛋白基因型分布及与相应多克隆抗体的反应特性

Distribution of porins genotypes and reactivity to polyclonal antibodies in 81 female patients with gonorrhea
下载PDF
导出
摘要 目的:采用PCR和探针技术分析临床淋病奈瑟菌野生株孔蛋白基因的基因型,并和相应多克隆抗体进行反应以检测基因突变对抗原性的影响。方法:采用PCR技术从淋病奈瑟菌野生株中扩增出特征性的孔蛋白基因片段,再用探针检测相应的高变区情况,以区分不同的准种(亚型),最后分别与原核表达的标准菌株孔蛋白多克隆抗体进行反应,观察相应的滴度。结果:孔蛋白基因型为A型的有35例,其余46例为B型。孔蛋白A型基因型变化较小,与抗体的反应性较一致;孔蛋白B型基因型变化稍大,但不影响与多克隆抗体的反应活性,A型与B型存在交叉反应,但活性较低,滴度<1∶40。结论:通过分析不同孔蛋白基因型淋病奈瑟菌与相应多克隆抗体的反应情况,有助于考察孔蛋白原核表达产物的生物学效应,对于人工合成出能针对绝大多数临床基因变异菌株的探针用于检测或治疗性抗体的制备有重要意义。 Objective:To analyze the genotype of the porin gene in Neisseria gonorrhoea wild strains. Bacterial agglutination has been carried out to verify whether there is an antigenicity change caused by the porin gene mutation.Methods:Porin gene was amplified by PCR from wild strains and probes were used to detect the mutation in VRs to differentiate the isoforms. Different strains reacted with the multi-clonal antibody to observe their titre.Results:There were 35 cases of type-A porin genotype and 46 cases of type B within the 71 cases. Greater mutations were found in type B than in type A with no effect on antigen-antibody reactivity. Cross reactivity existed between A and B with low titre(less than 1∶40).Conclusion:It must greatly help to inspect the biological effects of porins expressed by prokaryotic expression system in analyzing different porin genotype strains’ reactivity with antibody. It is also of significance to produce antibody with diagnostic or remedial potential aiming at most of strains of different porin genotypes
出处 《中国妇幼保健》 CAS 北大核心 2005年第13期1568-1570,共3页 Maternal and Child Health Care of China
基金 湖北省卫生厅资助项目(No.JX1B037)
关键词 淋病奈瑟菌 孔蛋白 基因型 多克隆抗体 Neisseria gonorrhoeae Porin Genotype Multi-clonal antibody
  • 相关文献

参考文献5

  • 1Turner CF. Untreated gonococcal and chlamydial infection in a probability sample of adults. JAMA, 2002, 287:726 ~733.
  • 2Van der Ley P. Topology of outer membrane porins in pathogenic Neisseria spp. Infect Immun , 1991, 59:2963 ~2971.
  • 3Trevor C, Fudyk. Genetic Diversity and Mosaicism at the por Locus of Neisseria gonorrhoeae . Journal of Bacteriology, 1999, 181 ( 18 ):5591 ~ 5599.
  • 4Durrie L, McKnew. Porin Variation among Clinical Isolates of Neisseria gonorrhoeae over a 10 - Year Period, as Determined by Por Variable Region Typing. The Journal of Infectious Diseases, 2003, 187: 1213 ~1222.
  • 5Heckels JE. The potential protective effect of immunization with outer membrane protein Ⅰ from Neisseria gonorrhoeae. J Gen Microbiol, 1989,135 (8): 2269~2276.

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部