参七汤抑制再灌注心肌细胞凋亡模型小鼠的实验研究

Empirical Study on the Inhibiting Effect of Shenqi Decoction on Myocardial Cell Apoptosis of Model Mice Induced by Reperfusion

目的:探讨中药参七汤抑制缺血再灌注心肌细胞凋亡的机制。方法:将小鼠随机分为参七汤(高、中、低剂量)组、消心痛对照组、空白对照组和模型组。参七汤组和消心痛组分别灌胃给药5d,空白对照组和模型组分别灌胃生理盐水5d,除空白对照组外,各组进行缺血再灌注造模,之后测定各组心肌组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)水平,分析心肌细胞凋亡指数和凋亡相关蛋白Bcl-2、Bax表达水平。结果:与模型组比较,参七汤组SOD活性升高、MDA水平降低,Bcl-2表达指数升高,Bax表达指数降低。结论:参七汤对心肌缺血和缺血后再灌注导致的心肌细胞损伤均具有较好的保护作用。

OBJECTIVE:To probe into the mechanism of Shenqi decoction on inhibiting apoptpsis of myocardial cell inˉduced by ischemic reperfusion.METHODS:The mice were randomly divided into Shenqi decoction group(high,middle and low dosage groups),isosorbide dinitrate control group,blank control group and model group.The Shenqi decoction group and isosorbide dinitrate group were respectively administered with drugs by lavage for5days while the blank control group and model group were respectively administered with normal sodium by lavage for5days.Except the blank control group the isˉchemic reperfusion model of each group was made,then the activity of superoxide dismutase(SOD),the level of malondiˉaldehyde(MDA)of cardiac muscle tissues were determined;the apoptotic index of myocardial cells and the expression of apoptosis-correlated protein Bcl-2and Bax were analyzed.RESULTS:Compared with the model group,the SOD activity and Bcl-2express index have been increased,but MDA level and bax express index have been decreased in Shenqi decoction group.CONCLUSION:Shenqi decoction does have a good protection effect on myocardial ischemia and myocardial cell injury induced by postischemia reperfusion.