摘要
以无籽西瓜黑蜜5号无菌苗子叶为外植体,对其离体培养及植株再生的条件进行了详细的研究。研究结果表明:子叶芽诱导的最佳培养基配方为MS+6.BA3.0mg/L+NAA0.1mg/L。芽的诱导需要先在黑暗条件下启动,生长分化则须在光下进行。适宜的生根培养基配方为1/2MS+IAA0.4mg/L和1/2MS+IBA0.3mg/L。试验初步建立了适用于无籽西瓜遗传转化和快速繁育的子叶再生系统。
The plantlet regeneration from Cotyledon of Citrullus lanatus was studied. The results showed that the most suitable medium for buds induction from cotyledon of Citrullus lanatus was MS + 6-BA 3. Omg/L + NAA 0. Img/L. The buds induction of the cotyledon should be started in darkness and its further growth and differentiation must be carried out in light. The optimum rooting media were 1/2MS + IAA 0. 4mg/Land 1/2MS + IBA 0. 3mg/L. The research preliminarily established a system of genetic transformation and quick propagation for cotyledon of Citrullus Lantus.
出处
《莱阳农学院学报》
2005年第2期118-121,共4页
Journal of Laiyang Agricultural College
基金
河南省杰出人才创新基金项目(200203056)
关键词
无籽西瓜
子叶
组织培养
植株再生
Citrullus Lantus
cotyledon
tissue culture
plantlet regeneration
