苯吸入染毒大鼠致多组织器官氧化损伤作用

Oxidation damage of benzene inhalation on multiple organs in rats

目的研究气态苯(106mg/m3)吸入染毒大鼠致组织器官氧化损伤作用,探讨苯对机体的脂质过氧化作用及机体的抗氧化损伤机制。方法通过建立气态苯动式吸入染毒大鼠的实验动物模型,连续染毒7d,每天4h;染毒结束后,采用分光光度法测定各组织器官(肺、脑、肝和外周血)中还原型谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSHPX)活力、超氧化物歧化酶(SOD)活性及脂质过氧化产物丙二醛(MDA)含量。结果苯吸入组大鼠肺、肝、脑组织及外周血均表现不同程度的氧化损伤,其中大鼠肺组织SOD活力,肝组织GSHPX和MDA及脑组织的MDA含量与对照组比较差异均有统计学意义(P<0.05),外周血GSH、GsHPx和MDA及TS0D水平与对照组比较也表现增加趋势。结论推测苯可诱发大鼠机体发生一系列的氧化应激损伤,并且具有多器官性的特点。

Objective To study the possible mechanisms of oxidative damages in rat multiple organs induced by gaseous benzene.Methods The mold of benzene inhalation in rats was built.The dose of inhalation was (106?mg/m^3) and the peirod was consecutive 7 days ((4?h/d)).After the inhalation toxicological experiment,the factors of oxidative damage of lung,brain,liver and peripheral blood were determined.Results Benzene inhalation caused the significant increase of the activity of superoxide dismutase(SOD)in lung,the activity of GSH-Px and the concentration of malonyl dialdehyde(MDA)in liver and the MDA in brain.At the same time,the level of reduced glutathione(GSH),GSH-PX,MDA and SOD in peripheral blood had the trend of increase.Conclusion Benzene may induce a series of oxidative damage in the body of the rat,and has the character of multiple organs.