牛磺酸与大鼠视网膜代谢型谷氨酸受体基因表达

Relationship between taurine and mRNA expression of metabotropic glutamate receptor subtype 6 in rat retina

目的探讨膳食添加牛磺酸大鼠在光照和暗环境中视网膜代谢型谷氨酸受体6亚型(mGluR6)的mRNA表达的相互关系。方法36只断乳大鼠随机分为3组:对照组、牛磺酸低剂量组及牛磺酸高剂量组(分别在饲料中添加0.3%和0.6%的牛磺酸),营养干预4周后,再随机分为光照组和暗环境组,继续喂养3d。用RTPCR技术检测光照和暗环境下大鼠视网膜mGluR6的mRNA表达。结果暗环境下mGluR6的mRNA表达显著高于光照环境。在光照及暗环境中牛磺酸高、低剂量组mGluR6的mRNA表达均较对照组增加,以光照状态下的增加更为明显;光照时,高剂量组的mRNA表达明显高于低剂量组;暗环境下,高、低剂量组之间差异无统计学意义。结论在光照及暗环境中,牛磺酸可明显增加大鼠mGluR6的mRNA表达,有助于其参与调节视网膜的视杆信号传递。

Objective To understand the relationship between taurine intake and mRNA expression of metabotropic glutamate receptor subtype 6 (mGluR6) in rat retinas in daylight and dim light.Methods 36 ablactation Wistar rats were divided into three groups:normal control,low and high dose taurine treatment groups(supplementation of 0.3% or 0.6% taurine per kilogram rat diet respectively).After 4 weeks,reverse transcription polymerase chain reaction (RT-PCR) was used to study mRNA expression of mGluR6 in rat retinas in daylight and dim light.Results mRNA expression of mGluR6 in dim light was higher than that in daylight.0.3% and 0.6% taurine markedly increased mRNA expression of mGluR6,especially in daylight.High dose taurine had stronger effect than low dose in daylight,but no remarkable difference was observed between 0.3% and 0.6% taurine treatment groups in dim light.Conclusion Taurine increases mRNA expression of mGluR6 in daylight and dim light in rat retinas,which helps taurine to modulate the rod visual signal transmission in rat.