人结肠高分化腺癌裸小鼠移植瘤模型的建立及其生物学特性

Establishment of a Transplantable Human Colon Well Differentiated Adenocarcinoma in Nude Mice and Study of Its Biological Characteristics

将人结肠高分化腺癌手术标本,接种于裸小鼠体内建成移植瘤THCN—8901,已传14代,历时2年多,生长稳定,可移植性成功率高.组织学及超微结构形态观察,均保持了原病人肿瘤的结构,并且有分泌功能,染色体分析证实为人染色体,众数在63～94之间,该瘤株具有稳定产生CEA的生物学特性,用^(111)In—CEAMcAb能使裸小鼠移植瘤清晰显像.此瘤株的建立为结肠癌癌变基础及实验性基因治疗研究,提供了较为理想的实验动物模型.

A transplantable human colon well differentiated adenocarcinoma being passed on in BALB/c nu/nu nude mice was established during a period of two years and subcutaneously for 14 generations. The Transplantabllity rate was 95. 6%. It was a well differentiated cancer and was histologically and ultrastructurally mirroring the transplanted tumor which retained the original structures of the human colon cancer as well as the secretory function. Chromosomal analysis of the tumor cells revealed a human chromosome aneuploidy pattern of up to 63-94 in number. These xenografts retained the function of secreting CEA. It is assumed that this serves as a useful and hopeful model for in vivo study of human colon adenocarcinoma.