细胞外基质在人系膜增生性肾小球肾炎病变中的变化

Changes in the extraccllular matrix in human mesangial proliferative glomerulonephritis

肾小球细胞外基质（尤其系膜基质）的过量聚积是肾小球硬化的主要变化之一。我们应用免疫酶标技术及计算机图像分析系统，检测了系膜增生性肾小球肾炎病变过程中细胞外基质的变化。结果表明：增生扩张的系膜基质中含有Ⅳ型胶原、层粘连蛋白、纤粘连蛋白等多种正常肾小球细胞外基质成分，并随病变进展明显增加。Ⅲ型胶原在中、重度系膜增生性肾小球肾炎时也出现于增多的系膜基质中，说明正常时不存在于肾小球的间质胶原在肾小球硬化过程中也起重要作用。

quantitative analysis of the components of glomerular extracellular matrix(ECM) was performed with the immunoperoxidase method(ABC method) and the computer imaging analysis system. The re-sults demonstrate that with the development of mesangial proliferative glomerulonephritis(MsPGN),normal components of glomerular ECM (type Ⅳ collagen,laminin, fibronectin)increased markedly. In addition, interstitial collagen (type Ⅲ collagen)appeared in the expanded mesangial matrix of moderate MsPGN and increased in severe MsPGN,suggesting that MsPGN is linked to the accumulation of ECM,not only the normal components,but also the interstitial collagen.