人原发性肝癌间质胶原及其mRNA的表达

Interstitial collagen distribution and its mRNA expression in human primary liver cancer

用ＡＢＣ方法对２９例人肝细胞癌和２例胆管细胞癌组织及癌旁肝组织Ⅰ型胶原、Ⅲ型前胶原进行免疫组化定位。结果显示两种胶原在细胞外分布是一致的。作者将胶原在肝细胞癌中的分布类型分为两大类：血管样分布和非血管样分布。癌组织内胶原呈血管样分布者，肿瘤易浸润生长及门静脉转移，其肝内转移灶发生率也显著高于非血管分布者，预后较差，推测胶原的分布形式可作为判断肝癌患者预后的一个指标。我们还用ｃＤＮＡ—ｍＲＮＡ原位分子杂交技术检测了本组病例中的２例正常肝及４例原发性肝癌、癌旁肝组织中Ⅰ型胶原基因的表达，结果提示人原发性肝癌组织中肿瘤细胞及间质细胞均可参与Ⅰ型胶原的合成、胶原合成可能与细胞的活跃增殖有关。

he localizatioii of type Ⅰ collagen and type Ⅲ procollagen in 29 cases of hepatocellular carcinoma(HCC)and 2 cases of cholangiocarcinoma as well as their surrounding tissues were studied with ABC method.The results showed that the distribution of these two types of collagen was similar in PLC as well as in its surrounding tissues.The distribution of interstitial collagen in HCC might be classified into two patterns by the authors’namely, the vasiform and non vascular distributions. The carcinoma with vasiform distribution pattern tends to grow infiltratively with much higher rate of portal venous embolism and intrahepatic metastasis developmeiit than that of the non vascular pattern(P<0.05). Employing the in situ hybridization technique for type Ⅰ collagen mRNA,the transcription of collagen mRNA occured not only in the interstitial cells but also in some ofthe liver carcinoma cells and giant tumor cells. No positlve signals of type Ⅰ collagen mRNA were noticed in the non tumorous sur- rounding tissues.