乙型肝炎失代偿期肝硬化患者血清内毒素、IL-12、IL-18水平的检测及其意义

Serum levels of endotoxin, IL-12 and IL-18 in patients with type B hepatitis-related decompensate cirrhosis

目的:探讨乙型肝炎肝硬化血清内毒素(LPS)、白介素-12(IL-12)、白介素-18(IL-18)水平变化及临床意义。方法:采用鲎试剂()-偶氮显色法、ELISA双抗体夹心法,分别测定80例肝硬化患者血清LPS、IL-12、IL-18水平。另设正常对照组(32例)。结果:与正常对照组比较,肝硬化患者血清LPS、IL-12、IL-18水平均明显升高,且随着肝功能越差,升高越明显(P<0.01);腹水组的LPS及IL-12、IL-18水平较无腹水组也明显增高(P<0.01)。结论:LPS、IL-12、IL-18均参与了乙型肝炎肝硬化的病理生理过程,并与病情的变化密切相关。检测这些指标有助于判断肝硬化患者的肝功能状况及疾病的严重程度。

Objective: To observe the changes of endotoxin, IL-12 and IL-18 levels in patients with type B hepatitis-related cirrhosis. Methods: Endotoxin, IL-12 and IL-18 contents in 80 patients with type B hepatitis-related cirrhosis and 32 normal controls were determined by limulus test and ELISA, respectively. Results: The levels of endotoxin, IL-12 and IL-18 were significantly higher in cirrhosis group than those in control group, especially in patients with decompensate cirrhosis (P<0.01). Conclusion: Endotoxin, IL-12 and IL-18 are involved in the pathophysiology of type B hepatitis-related cirrhosis, and their detection is helpful in judging the disease severity.