摘要
应用EB病毒DNABamHIW片段、EBNA一2A、B型分了探针,检测我区鼻咽癌(包括鼻咽原位癌)、临床高度怀疑鼻咽癌(包括鼻咽上皮细胞非典型增生、颈淋巴结肿大及鼻咽结节)和慢性鼻咽炎等活检组织共96例。各组均以A型病毒感染为主:鼻咽癌组阳性为27/35(77.1%);临床疑癌组7/10(70.0%);慢性鼻咽炎织18/27(66.7%)。W和A型均阳性的鼻咽癌组为20/29(69.0%);非癌组为8/25(32.0%)。同时用抗补体免疫荧光法检测EBNA的表达,鼻咽癌组阳性为19/20(95.0%),而慢性炎组为1/15(6.7%)。以EB病毒DNABamHIW片段、EBNA一2A型片段和EBNA表达检测中任一项阳性作为EB病毒感染、整合或表达的依据,则鼻咽癌组与非癌组之间有明显的差别(P<0.001)。这从另一角度提示EB病毒(特别是EBNA一2A型)感染与我区鼻咽癌病因、发病有密切关系。
asopharyngeal biopsies from 96 cases of clinically suspected nasopharyngeal carcinoma and chronic nasopharyngitis were investigated using EB virus DNA BamHI W fragment and EBNA一2A,Btype probes.There was a significant dominance of EBV EBNA一2 A type infection on all samples:nasopharyngeal carcinoma(NPC) 27 / 35 (77.1%), clinically suspected NPC 7 / 10(70.0%),chronic nasopharyngitis(CNP) 18/ 27 (66.7%) .Both W and A probes positive were 20 /29(69.0%) in NPC and 8/ 25(32.0%) in non一NPC, indicating a close relationship between the infection ofEBNA一2A type virus and NPC in this series. Meanwhile, the expression of EBNA on 35 cases of biopsy were detected using the anti一complement immunofluorescence method,19 / 20 (95.0%) were positive in the NPC group and 1 / 15(6.7%) in the non一NPC group.Based on the positive results in detection ofA,W or EBNA expression,there was a significant difference between NPC and non一NPC groups(P<0.001).It is suggested that EBV infection, especially type A,plays an important role in the etiology and pathogenesis of NPC in the Zhanjiang are.
出处
《中华病理学杂志》
CSCD
北大核心
1994年第4期200-203,T041,共4页
Chinese Journal of Pathology