良、恶性鼻咽活检组织中EB病毒DNA检测、EBNA的分型及表达

Investigation of EB virus DNA BamHI W fragment, EBNA-2 types and EBNA expression in benign and malignant nasopharyngeal biopies

应用ＥＢ病毒ＤＮＡＢａｍＨＩＷ片段、ＥＢＮＡ一２Ａ、Ｂ型分了探针，检测我区鼻咽癌（包括鼻咽原位癌）、临床高度怀疑鼻咽癌（包括鼻咽上皮细胞非典型增生、颈淋巴结肿大及鼻咽结节）和慢性鼻咽炎等活检组织共９６例。各组均以Ａ型病毒感染为主：鼻咽癌组阳性为２７／３５（７７．１％）；临床疑癌组７／１０（７０．０％）；慢性鼻咽炎织１８／２７（６６．７％）。Ｗ和Ａ型均阳性的鼻咽癌组为２０／２９（６９．０％）；非癌组为８／２５（３２．０％）。同时用抗补体免疫荧光法检测ＥＢＮＡ的表达，鼻咽癌组阳性为１９／２０（９５．０％），而慢性炎组为１／１５（６．７％）。以ＥＢ病毒ＤＮＡＢａｍＨＩＷ片段、ＥＢＮＡ一２Ａ型片段和ＥＢＮＡ表达检测中任一项阳性作为ＥＢ病毒感染、整合或表达的依据，则鼻咽癌组与非癌组之间有明显的差别（Ｐ＜０．００１）。这从另一角度提示ＥＢ病毒（特别是ＥＢＮＡ一２Ａ型）感染与我区鼻咽癌病因、发病有密切关系。

asopharyngeal biopsies from 96 cases of clinically suspected nasopharyngeal carcinoma and chronic nasopharyngitis were investigated using EB virus DNA BamHI W fragment and EBNA一2A,Btype probes.There was a significant dominance of EBV EBNA一2 A type infection on all samples:nasopharyngeal carcinoma(NPC) 27 / 35 (77.1%), clinically suspected NPC 7 / 10(70.0%),chronic nasopharyngitis(CNP) 18/ 27 (66.7%) .Both W and A probes positive were 20 /29(69.0%) in NPC and 8/ 25(32.0%) in non一NPC, indicating a close relationship between the infection ofEBNA一2A type virus and NPC in this series. Meanwhile, the expression of EBNA on 35 cases of biopsy were detected using the anti一complement immunofluorescence method,19 / 20 (95.0%) were positive in the NPC group and 1 / 15(6.7%) in the non一NPC group.Based on the positive results in detection ofA,W or EBNA expression,there was a significant difference between NPC and non一NPC groups(P<0.001).It is suggested that EBV infection, especially type A,plays an important role in the etiology and pathogenesis of NPC in the Zhanjiang are.