胸腔内类癌伴Cushing综合征的病理学观察

Pathological study on thoracic carcinoids accompanied with Cushing’s syndrome

报道８例胸腔内类癌（肺５例、胸腺３例）伴Ｃｕｓｈｉｎｇ综合征病例。年龄１３～５３岁，平均３２．９岁。组织学证实为典型类癌。免疫组化神经特异性稀醇化酶（ＮＳＥ）、嗜铬蛋白Ａ（ＣｇＡ）、ＡＣＴＨ均显强阳性反应。一些肿瘤Ｓ－１００、降钙素，ＨＣＧα和ＨＣＧβ亦显阳性反应。电镜检查：所有肿瘤细胞均有不等数量的神经分泌颗粒；免疫电镜示：分泌颗粒中有标记ＡＣＴＨ金颗粒；原位杂交：在肿瘤细胞内有ＣｇＡｍＲＮＡ的过度表达。

ight patients with carcinoid tumors(5 from the lungs and 3 from the thyrnus)associated with Cushing’s syndrome were studied.Among them, 5 were male and 3 female with a mean age of 32.5 years.Histologically,all were typical carcinoids, Immunohistochemically,tumor cells were strongly positive of NSE, chromogranin A and ACTH in all 8 cases. Some tumors also positive for S-100, calci-tonin, HCG_α and HCG_β.Various amounts of neurosecretory granules were found in the tumors by elec-tron microscopy and ACTH positive dots were also noticed in some of the neurosecretory granules in the tumor cells by immunoelectron microscopical colloidal gold labelling technique. By in situ hybridization technique,overexpression of chromogranin A mRNA was detected in all of the tumors reported.