鼻咽癌活检组织中HLA、EBNA及间质中T淋巴细胞亚群的分布

The Expression of HLA, Epstein-Barr Virus Nuclear Antigen and the Infiltration of T Lymphocyte Subsets in Nasopharyngeal Biopsy Tissues

本实验对人类白细胞抗原（ＨＬＡ）、爱波斯坦－巴尔病毒核抗原（ＥＢＮＡ）、Ｔ细胞亚群的表达及分布进行综合分析，探讨其在癌变过程中的作用。用单克隆抗体Ｗ＿６／３２（抗ＨＬＡ－Ａ，Ｂ，Ｃ）、ＣＲ＿３／４３（抗ＨＬＡ－ＤＲ，ＤＰ，ＤＱ）、和Ｔ＿４、Ｔ＿８、Ｔ＿（１１），以碱性磷酸酶抗碱性磷酸酶法（ＡＰＡＡＰ）和用鼻咽癌患者血清以抗补体Ｃ＿３免疫荧光法，检测鼻咽癌（２５例）及慢性鼻咽炎（１０例）活检组织中ＨＬＡ、Ｔ细胞亚群分型和ＥＢＮＡ的表达。结果显示，鼻咽癌癌细胞多有ＨＬＡ－Ⅰ和Ⅱ型抗原表达，而以ＨＬＡ－Ⅱ型抗原表达为明显；慢性鼻咽炎上皮细胞表达ＨＬＡ－Ⅰ和Ⅱ型抗原较弱，与癌细胞比较，差异有显著性（Ｐ＜０．０５）。提示在癌变过程中，存在着ＨＬＡ表达的异常，体内ＥＢ病毒特异性Ｔ细胞可能不能识别带ＨＬＡ－Ⅱ型抗原的癌细胞，因而使其逃脱了机体的免疫监视。鼻咽癌组织中，癌细胞ＥＢＮＡ呈阳性和强阳性；癌间质中Ｔ细胞总数、Ｔ辅助、Ｔ杀伤细胞数量均减少，证明鼻咽癌局部细胞免疫功能降低。

The HLA typing,EBV-EBNA expression and the infiltration of T cell subsets innasopharyngeal biopsies were investigated using APAAP(Alkaline phosphatase and anti-alkalinephosphatase)and anti-complement immunofluorescence method with a panel of monoclonal antibodieswhich are W_6/32(anti -NLA-A,B,C),CR_3/43(anti-HLA-DR,DP,DQ),serum from nasopharyngeal carcinoma(NPC, anti-EBNA) and T_4,T_8, T_(11)(anti-Th,Tc, T_3,respectively).There were25 cases of NPC, 10 of chronic nasopharyngitis(CNP),CNE-2Z cell line and transplants of CNE-2Z,NCN-Z-1 and fetal nasopharynx mucosa under detection. The results showed that the tumor cells ofNPC expressed not only HLA-Ⅰbut also HLA-Ⅱ.The expression of HLA-Ⅱ was stronger than that ofHLA-Ⅰin NPC. The expression of HLA-Ⅰand Ⅱ in epithelial cells of CNP was weak.There was a signif-icant distinction between those groups(P<0.05). The expression of HLA-Ⅰ,Ⅱ was the strongest inCNE-2Z smears and transplants in under mice of CNE-2Z, NCN-Z-1, both negative in fetalnasopharyngeal epithelial transplants.It suggested that HLA express abnormally during carcinogenesis,the cancer cells with HLA-Ⅱ be not recognized by EBV specific T cells,hence escape from the immunesurveillance. Also it demonstrated that the EBNA expression in the cancer cells of NPC was strongly pos-itive,the cells of T_3,Th,and Tc in NPC were decreased.It needs further study whether or not the EB vi-rus infection can induce HLA phenotype changes in tumor cells and what the biological functions of HLA-Ⅱ expression in tumor cells are.