电子显微镜与聚合酶链反应技术检测阴道及子宫颈人乳头瘤病毒

Detection of vaginal and cervical human papil-lomavirus by electronic micraseope examinationand polymerase chain reaction technique

应用电子显微镜（电镜）、聚合酶链反应（ＰＣＲ）技术，对１９９０年４月至７月，因外阴瘙痒、白带多就诊检查发现阴道下１／３或子宫颈有异常突起，病理及核酸分子杂交技术检查证实为人乳头瘤病毒（ＮＰＶ）感染的标本４７份，进行ＨＰＶ检测。ＰＣＲ检测阴道尖锐湿疣ＨＰＶ６，１１的阳性率为９２．６％，ＨＰＶ６，１１和ＨＰＶ１６，１８混合感染阳性率为３．７％。电镜下未观察到典型的ＨＰＶ颗粒，观察到挖空细胞核内存在着染色质之间颗粒及染色质周围颗粒，在３例斑点杂交ＨＰＶ１１阳性者的标本中观察到核内小体。基底细胞也有改变。

orry-seven specimens,biopsied during April～July 1990,pathologically proven to be vaginal or cervi-cal condvloma acuminata(CA)in 34, wart-like lesionsin 6,infections in 7,were screened for detection of hu-man papillomavirus(HPV)by electronic microscope(EM) examination and polymerase chain reaction(PCR)technique,HPV type 11(HPV-11)infectionswere diagnosed by Dot-blot hybridization in 40 out ofthese 47 specimens。 Results:(1) No typical virus parti-cles was observed in all samples under EM examina-tions. However,interchromatin and perichromatingranules were found within the nuclei of koilocytes in 88.23%of the vaginal CA specimens. Nuclear bodieswere seen in 3 out of 27 vaginal CA and HPV11 positivespecimens. Furihermore,koilocytosis was also seen in the basal and parabasal layers of vaginal mucosa,(2)The positive rate for detection of HPV11 and HPV 6DN A by PCR technique in vaginal CA specimens was92.6%, while positive rate for HPV 11,6,16 and 18DNA was only 3.7%。