软骨肉瘤与骨肉瘤免疫组化研究

An Immunohistochemical Study of Chon-drosarcomas and Osteosarcomas

应用免疫组化方法对３６例各亚型软骨肉瘤、骨肉瘤以及骨Ｅｗｉｎｇ氏瘤、淋巴瘤各５例，进行了Ｓ－１００蛋白（Ｓ－１００）波形蛋白（ｖｉｒｎｅｎｔｉｎ，Ｖｉｍ）、α＿１－抗胰糜蛋白酶（ａｌｐｈａ１－ａｎｔｉｃｈｙｍｏｔｒｙｐｓｉｎ，ＡＡＣＴ）、溶菌酶（ｌｙｓｏｚｙｍｅ，Ｌｙｓｏ）、白细胞共同抗原（ｌｅｕｃｏｃｙｔｅｃｏｍｍｏｎａｎｔｉｇｅｎ，ＬＣＡ）、Ｌｅｕ７、ｏｓｔｅｏｎｅｃｔｉｎ（ＯＮ）、ＮＳＥ等八种免疫抗血清的检测，结果发现，Ｓ－１００、Ｌｙｓｏ可以作为成软骨性肿瘤瘤细胞的标记物，ＯＮ可以作为骨细胞骨母细胞样分化的标记物之一。作者讨论了各亚型软骨肉瘤、骨肉瘤的组织发生学，也探讨了间叶性软骨肉瘤与骨的Ｅｗｉｎｇ氏瘤、骨的淋巴瘤的鉴别诊断问题。

Abstract Immunohistochemical Studies were performed on 21chondrosarcomas and 1 5 osteosarcomas,includipg sub-types. Five Ewing,s tu mors and 5 lymphomas were alsosimilarly studied,The immunoreactivity of 46 tumors wasstudied using antlsera for S-100 proteln(S-100),vimentin( Vim ),alphW-1-antichymotrypsin(AACT),lysozyme(Lyso),leucocyte common antigen(LCA), leu 7,os-teonectin(ON)and NSE. Astrong reactivity for Vim wasf0und血tumO,cek0fch0ndroosarcomas, osteosarcomas and Ewing's tumors.Staining for S- 100,Lyso were consis-tently p‘Sitive in chondrocytes of chondrosarcomas and os-teosarcomas. S-100,Lyso seem to be a marker for tumorsof CartilagiOnous origin and differentiation,ON seems alsoto be a differentiation marker of osteoblasts.