乙型肝炎病毒前S_1蛋白与HBV-DNA水平和HBV标志物及肝脏功能的相关性研究

Correlation Studies on Hepatitis b Virus-pre-S_1 Protein and HBV DNA, Standard HBV Markers,Hepatic Function in Patients with Hepatitis b

目的:探讨检测乙型肝炎病毒前S1蛋白(Pre-S1)的临床意义。方法:本文用ELISA法对160例慢性乙型肝炎患者进行Pre-S1蛋白及HBV标志物检测,同时用荧光定量PCR(FQ-PCR)法定量检测HBV-DNA。结果:HBsAg、HBeAg、抗-HBc阳性者52例,HBV-DNA与Pre-S1蛋白的检出率分别为96.2%和94.2%;HBsAg、抗-HBe和抗-HBc阳性者88例,HBV-DNA与Pre-S1蛋白的检出率分别为75.0%和69.3%;HBsAg和抗-HBc阳性者15例,HBV-DNA与Pre-S1蛋白的检出率分别为60.0%、53.5%;5例单独抗-HBc阳性者,HBV-DNA与Pre-S1蛋白检出率均40.0%。127例HBV-DNA阳性患者HBeAg、Pre-S1蛋白的检出率分别为39.4%(50/127)、93.7%(119/127);两者与HBV-DNA的总符合率分别为50.6%(81/160)、94.4%(151/160)。Pre-S1蛋白与HBeAg检出率差异有显著性(χ2=58.13,P<0.01);HBV-DNA与Pre-S1蛋白检出率差异无显著性。Pre-S1蛋白阳性患者ALT和AST水平阴性者高,但差异未见显著性(P>0.05)。结论:Pre-S1蛋白和HBV-DNA相关较大,能反映HBV复制,有可能作为体内HBV复制的实验室指标。

Objective To investigate the correlationstudies on hepatitis b virus-pre-S_1 protein and HBV DNA,standard HBV markers, hepatic function in patients with hepatitis b.Methods Sample collected from 160 chronic hepatitis B patients were analyzed in the study.The HBV Pre-S_1 protein (Pre-S_1) and standard HBV markers (HBsAg,Anti-HBS,HBeAg,Anti-HBE and Anti-HBc) were analyzed by (ELISA).While HBV DNA (polymerase region) was analyzed by fluorescence quantitative polymerase chain reaction (Fq-PCR).Results The positive rates of HBV-DNA and Pre-S_1 protein in 52 patients with HBsAg,HBeAg and anti-HBc(+) were 96.2%,94.2%,respectively.The positive rates of HBV-DNA and Pre-S_1 protein in 88 patients with HBsAg,anti-HBe and anti-HBc(+) were 75.0%,69.3%,respectively.The positive rates of HBV-DNA and Pre-S_1 protein in 15 patients with HBsAg and anti-HBc(+) were 60.0%,53.5%,respectively.The positive rates of HBV-DNA and Pre-S_1 protein in 5 patients with only anti-HBc(+) were 40.0%.The positive rate of HBeAg and Pre-S_1 were 39.4%(50/127),93.7%(119/127) in 127 patients with HBV-DNA(+).The concordance rate between HBeAg and HBV-DNA was 50.6%(81/160);the concordance rate between Pre-S_1 and HBV-DNA was 94.4%(151/160). activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly strengthened in patients with positive Pre-S_1 protein,but were not significant (P>0.05).Conclusion From the study,the testing result of Pre-S_1 protein and HBV-DNA is highly correlated.Both methods can reflect the replication of HBV in patients.But the operation procedure of Pre-S_1 is simple and does not require skillful technician.Therefore,Pre-S_1 should be more suitable in daily routine testing.