强直性脊柱炎患者抗组蛋白抗体测定及其临床意义

DETECTION OF SERUM ANTI-HISTONE ANTIBODIES IN PATIENTS WITH ANKYLOSING SPONDYLITIS AND ITS CLINICAL SIGNIFICANCE

以酶联免疫吸附试验（ＥＬＩＳＡ）及免疫印染技术对７８例强直性脊柱炎（ＡＳ）患者的血清进行抗总组蛋白及组蛋白亚单位抗体测定。发现抗，总组蛋白抗体（ＡＨＡ）水平在ＡＳ组显著高于正常对照组及痛风性关节炎组，在活动期ＡＳ组高于非活动期组，其阳性率达２８％在有虹膜炎的ＡＳ组显著高于无虹膜炎组；抗Ｈ＿３抗体与虹膜炎有显著性关联。结果提示高水平ＡＨＡ和Ｈ＿３抗体与ＡＳ病情活动及与ＡＳ并发虹膜炎有关。

erum anti一total and subtypical histone antibodies were deteeted with ELISA and Western一Blotting in 78 ankylosing spondylitis(AS)and 38 gouty arthritis fGA) patients as well as in 45 normal subjects. The re-sults showed that the positive rate of anti一histone antibodies(AHA)was about 5% in nonnal subjects or GA patients and 24. 4% in AS patients.The mean level of AHAs was much higher in AS group than those in the controls.IgG-and lgM-AHA levels in AS patients during active stage were higher than in those duringinactive stage. AHA Ievels were much more elevated in AS patients with present illness or past history of uveitis than in those wlthout. The high levels of AHAs(espoially anti一H_3 antibody) were associated with uveitis in AS patients.The results suggested that the high level of AHAs might be a monitoring parameter for disease activity and occurrence ofuveitis in patients with AS.