性激素对豚鼠表皮郎格罕细胞及变应性接触性皮炎的影响

Effect of Sex Hormones on Epidermal Langerhans Cells and Allergic Dermatitis in Guinea Pigs

观察了性激素对豚鼠表皮郎格罕细胞及变应性接触性皮炎（ＡＣＤ）的影响。五组豚鼠分别皮下注射麻油、雌二醇、黄体酮、丙酸睾丸酮及外用丙酸睾丸酮。二周后作表皮ＬＣ计数。用两酸睾丸酮的两组豚鼠表皮ＬＣ数较前三组明显减少（ Ｐ ＜ ０． ０１）。同时各组动物用 ＤＮＣＢ致敏。激发前一周各组仍继续使用上述药物。二周后激发ＡＣＤ，丙酸睾丸酮的两组皮肤反应以及真皮内单一核细胞浸润较另三组明显轻微（ Ｐ ＜０．０１），提示丙酸睾丸酮可抑制豚鼠的 ＡＣＤ。

We studied the effect of different sex hormones on epidermal Langerhans cells (LC) and DNCB-induced allergic contact dermatitis (ACD). 25 guinea pigs were randomly divided into 5 groups which received respectively subcutanous injection of sesame oil (group 1 ), estradiol (group 2 ), progesterone (group 3 ), testosterone propionate (group 4 ), and topical application of testosterone propionate (group 5)once daily for 2 weeks. Then,skin biopsies were taken and the number of LC was counted,LC densities in groups 4 and 5 were significantly lower than those of group 1, 2 and 3 (P <0. 01 ). At the time of skin biopsy,all 5 groups of guinea pigs were sensitized with 2% DNCB, and continued to receive sex hormones and sesame oil for 7 days. Two weeks after sensitization, all groups were challenged with 0. 1% DNCB. 24 hours after challenge skin reactivity and dermal mononuclear cell responses were assessed, both were significantly lower in groups 4 and 5 than those in groups 1,2 and 3 (P <0. 01). We conclude that systematic and topical use of testosterone propionate can inhibit DNCB--induced ACD.