聚合酶链反应对53例皮肤结核病和结节性皮肤病的研究

Study of 53 Cases of Cutaueous Tuberculosis and Nodular Dermatoses by Polymerase Chain Reaction

采用聚合酶链反应（ＰＣＲ）从临床诊断或病理诊断为皮肤结核病（ＣＴ）或结节性皮肤病（ＮＤｓ）的５３份石蜡包埋组织中检测结核杆菌ＤＮＡ。ＰＣＲ阳性率在临床诊断为ＣＴ和ＮＤｓ者中分别为４５％和４７％。ＰＣＲ与临床诊断的一致率为４７％。ＰＣＲ阳性率在病理诊断为ＣＴ和ＮＤｓ者中分别为４２％和５３％。ＰＣＲ与病理诊断的一致率为４３％。以上结果提示皮肤结核病和结节性皮肤病在临床和病理上都不易区别，辅以ＰＣＲ技术有助于从皮损中找到结核杆菌感染的病原学证据。

Mycobacterium tuberculosis DNA was detected by polymerase chain reaction(PCR) from 53 samples of paraffin embedded tissues which were clinically or pathologically diagnosed as cutaneous tuberculosis (CT) or nodular dermatoses (NDs). The positive identification rates by PCR in clinically diagnosed CT and NDs patients were 45 % and 47%, respectively. The consistency in diagnosis between PCR and the clinical diagnosis was 47%. The positive identification rates by PCR in pathologically diagnosed CT and NDs patinets were 42% and 53 %,respectively. The consistency in diagnosis between PCR and the pathological diagnosis was 43%. These results indicate that it is difficult to successfully differentiate between cutaneous tuberculosis and nodular dermatoses either clinically or pathologically, and that the PCRmethod is useful to detect pathogenic evidence in the lesions of tuberculous infection.