摘要
采用聚合酶链反应(PCR)从临床诊断或病理诊断为皮肤结核病(CT)或结节性皮肤病(NDs)的53份石蜡包埋组织中检测结核杆菌DNA。PCR阳性率在临床诊断为CT和NDs者中分别为45%和47%。PCR与临床诊断的一致率为47%。PCR阳性率在病理诊断为CT和NDs者中分别为42%和53%。PCR与病理诊断的一致率为43%。以上结果提示皮肤结核病和结节性皮肤病在临床和病理上都不易区别,辅以PCR技术有助于从皮损中找到结核杆菌感染的病原学证据。
Mycobacterium tuberculosis DNA was detected by polymerase chain reaction(PCR) from 53 samples of paraffin embedded tissues which were clinically or pathologically diagnosed as cutaneous tuberculosis (CT) or nodular dermatoses (NDs). The positive identification rates by PCR in clinically diagnosed CT and NDs patients were 45 % and 47%, respectively. The consistency in diagnosis between PCR and the clinical diagnosis was 47%. The positive identification rates by PCR in pathologically diagnosed CT and NDs patinets were 42% and 53 %,respectively. The consistency in diagnosis between PCR and the pathological diagnosis was 43%. These results indicate that it is difficult to successfully differentiate between cutaneous tuberculosis and nodular dermatoses either clinically or pathologically, and that the PCRmethod is useful to detect pathogenic evidence in the lesions of tuberculous infection.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1994年第5期273-275,T002,共4页
Chinese Journal of Dermatology
基金
四川省卫生厅科研基金
关键词
结节性
皮肤病
聚合酶链反应
结核病
Tuberculosis,cutaneous Nodular dermatoses Polymerase chain reaction