弱视猫视觉系统三级神经元及其突触的超微结构研究

Ultrastructural studies of neurons and synapses in thevisual system of amblyopic cats.

以２～４周龄幼猫制作单眼剥夺弱视模型，对比制作术前及饲养１２周后的图形视觉诱发电位（ＰＶＥＰ）改变，证实产生弱视后，灌注固定幼猫。在透射电镜下观察剥夺眼视网膜中央区神经节细胞、外侧膝状体和视皮质神经元及其突触的超微结构，发现三级神经元及突触均有不同程度的退变和萎缩。主要表现为神经元核皱缩、变形，线粒体皱缩、嵴紊乱，基质电子密度增高，高尔基体减少、不发达，粗面内质网解聚，可见吞噬体、脂褐素及指纹状结构，突触发育不良、畸形变等。本实验表明，弱视产生有其亚细胞病理学基础，生后早期视紊乱可导致视觉系统的组织病理损害。

Abstract14 kittens of 2-4 weeks were randomly divided in-to normal(N)and monocular deprivation(MD)groups.PVEP was tested before and after monoculardeprivation by blepharorrhaphy for 12 weeks,whendevelopment of amblyopia was confirmed.UnderTEM,the central retinal ganglion cells,the ipsilateralgeniculate nucleus layers,the neurons of layer IV ofvisual cortex area 17,and the neural synapses of MDand N eyes were examined to find that atrophic anddegenerative changes to various degrees existed in theMD eyes,supporting the authors belief that the func-tional changes in amblyopia were based on subcellularpathology,and visual disorders after birth resulted inhistopathological injuries to the visual system.