角膜神经损伤后再生的形态和功能学研究

Morphological and functional studies on nerve regen-eration after corneal nerve injuries.

采用氯化金神经组织染色法与辣根过氧化物酶逆轴浆运输法相结合，对３６只兔角膜缘穿透性切开和穿透性角膜移植术后６个月角膜神经再生情况进行动态观察。结果：１８０°角膜缘穿透性切开术后１个月，可见角膜缘细小神经分支的芽生；术后２个月，有１～２支神经再生进入角膜基质；术后６个月，有少数基质神经再生。自体穿透性角膜移植和同种异体穿透性角膜移植术后神经的再生差异无显著性。在穿透性角膜移植术后６个月，三叉神经节（ＴＧ）中标记神经元的数量仍未达到正常水平。结果表明，在角膜神经完全损伤后６个月，角膜神经再生并不能恢复正常神经密度和功能。

AbstractUsing gold chloride impregnation of nerves andhorse-radish peroxidase(HRP) axoplasrna retro-grade tracing technique,we monitored nerve regenera-tion over a period of 6 months following penetratingperilimbal incisions and penetrating keratoplasties(PKHin rabbits. Post-operatively, at 1 month aftera180°perilimbal incision,loose unconnected subep-ithelial plexus were present in the limbus,at 2 months1-2bundles of deep stromal nerve were seen in thestroma and by 6months only a few stromal nerves re-generated. There was no difference in nerve regenera-tion between post-operative autograft and allograftPKP. By 6 months,the quantity of HRP-labelled.cells in the trigeminal ganglia was less than the normal level. The results indicated that nerve regeneration by6 months after corneal nerve injuries was inadequate torestore a normal corneal nerve extent and function.