肝素抑制后发性白内障形成的细胞学研究

A cytological study of inhibition of secondary cataractwith heparin

后发性白内障主要是白内障囊外摘除术后后囊膜上成纤维细胞的迁徙和增殖所致。我们用细胞培养的方法，通过细胞生长周期测定、细胞ＤＮＡ含量测定、细胞增长数及密度观察等，研究肝素对成纤维细胞生长的抑制作用，发现用肝素后成纤维细胞生长缓慢，肝素抑制成纤维细胞生长的抑制点在细胞周期的Ｇ＿１期和Ｓ期之间以及Ｍ期。

AbstractSecondary cataract is caused primarily by migra-tion and proliferation of fibroblasts on the posteriorcapsular surface after extracapsular cataract extraction。Human fetus skin fibroblast cell culture wasused,by measurement of cell s life cycle,cellularDNA content ,increase of cell number and density,toinvestigate the heparin inhibitory action on the growthof the cells. It is discovered that the growth rate of thefibroblasts is lowered in the culture medium with he -parin and the inhibition takes place at the interval be-tween G_1 and S stage and at M stage of the cell’s lifecycle.