EB病毒与干燥综合征发病相关性的研究

Intcrrelationship between EB virus and Sjogrcn'ssyndrome

为了进一步证实ＥＢ病毒与干燥综合征发病的关系，作者设计了ＥＢ病毒ＢａｍＷ片段特异的引物和寡核苷酸探针，利用ＰＣＲ体外基因扩增方法和核酸分子杂交技术检测出干燥综合征患者外周血白细胞及唇腺组织中存在有高频率的ＥＢ病毒ＤＮＡ，而对照组的阳性率相对很低。用原位杂交方法检测干燥综合征患者唇腺组织中ＥＢ病毒ＤＮＡ，共２８例中有１６例为阳性。结果说明干燥综合征患者ＥＢ病毒感染率较正常人明显增高，且ＥＢ病毒感染与靶器官的损害有关，提示靶器官控制ＥＢ病毒细胞内复制的免疫机制出现异常。

e designed a specific primer of EB virus Bam Wfragment and EBV DNA sequence by using thepolymerase chain reaction (PCR) to amplify the EBVDNA sequences and then performing thehybndization analysis (dot blot and Southern transfer)with a end-labelled internal oligonucleotideprobe. There was significantly higher positive rate of24% (7/29) for the labial glands of SJbgren'ssyndrome patients than 68% (20/29) for p Bam Wprobe. In situ DNA hybridization, with (a35S) DCTPlabelled EBV DNA probe, the EBV genomes weredemonstrated in the labial glands of the Suogren'ssyndrome patients (l6/28). Moreover. the elevatedcontent of EBV DNA was identified in those withmore severe destruction of labial glands. We concludethat EBV has a normal site of latency at saliveryglands in a nonpathogenic state, and may be reactivat-ed in SJtigren's syndrome patients and play a role inthe pathogenesis on this disease. The great sensitivityof PCR and the ability to analyze very small tissuebiopsies (fresh or paraffin embedded) make this tech-nique applicable to clinical diagnosis.