谷氨酸受体在大鼠c－foS基因的表达及其与癫痫易感性的关系

Roles of glutamate receptor in c-fos gene expressionand epilepsy susceptibihty in rats

为探讨Ｎ一甲基一Ｄ一天冬氨酸（ＮＭＤＡ）受体在ｃ一ｆｏｓ基因表达及其与癫痫易感性的关系，我们以Ｎｏｒｔｈｅｒｎ印迹杂交法测定结果显示：（１）外源性ＮＭＤＡ可诱导脑细胞ｃ一ｆｏｓ基因的ｍＲＮＡ表达；竞争性或非竞争性ＮＭＤＡ受体拮抗剂可完中阻断ｃ一ｆｏｓ基因ｍＲＮＡ表达。（２）ＮＭＤＡ诱导脑内ｃ一ｆｏｓ的ｍＲＮＡ表达随神经元发育呈上升趋势，于体外培养２４天达高岭。（３）在发育中，听源性癫痫易感大鼠脑细胞的ｃ一ｆｏｓ基因ｍＲＮＡ表达均高于对照组，且在细胞培养１８天时差异有显著意义。提示ＮＭＤＡ受体活性的变化可能与癫痫易感性的形成有关。

e use NMDA to induce expression of c-fosmRNA as a marker to observe the activity ofNMDA receptor in neurons during development, andcompare the activity of NMDA receptor betweenaudiogenic epilepsy -prone (P77PMC) andaudiogenic epilepsy resistant rats brain. In primaryculture of rats cerebral cortical neurons NMDA in-duced c-fos mRNA expression exhibites dose andtime-dependent changes, which can be prevented byantagonists. During the development of neurons, theNMDA -induced c-fos mRNA expression reaches amaximum at day 24. NMDA-induced c-fos mRNAexpression of P77PMC rats is higher than that of con-trols during 6 to 24 days in vitro with significant difference (P<0.05) at day 18. To present changes incfos mRNA expression induced by NMDA in cul-tured P77PMC rat cortical neurons may be one ofthe factors related to susceptibility of epilepsy inP77PMC rats