DNA结合蛋白在正常健康个体与癌症病人之间的不同表现

DIFFERENT MANIFESTATION OF DNA BINDING PROTEIN IN HEALTHY INDIVIDUAKS AND CANCER PATIENT

我们将分离６４－ＤＰ的方法加以改进，首次分离到电泳纯的ａｌｐｈａＤＮＡ结合蛋白（简称αＤＢＰ），用此制成羊抗αＤＢＰ，用环状免疫单扩散法对上千例各类癌症病人和正常人血清作了测定。发现正常健康的个体其血清中αＤＢＰ含量既丰富又均一，同癌症病人所呈现的不均一性形成强烈的反差。提示αＤＢＰ由均一变成不均一可能是体内出现癌变的一个征象。

Abstract By a modified serum 64-DP isolation method we successfully isolated α-DNA Binding protein( αDBP)to electrophoretic purity. Analysis by SDS-PAGE revealed a molecular weight of 59000.Itsuggested that αDBP is a glycoprotein. Goat anti-αDBP anti-serum was prepared and single radial immunodiffusion assay was used to screen 256 healthy individuals(teachers, students, workers andpeasents)and serum samples from 969 patients with various kinds of cancers. Contrary to previous find-ings,we found that serum αDBP was abundant in healthy individuals with homogeneous precipitation rigs,and was significantly increased in the serum of cancer patients. However, it depicted a heterogeneous pattern with 1～4 rings of various thickness. This phenomenon was observed in 94.2%of patients with liver cancer regardless of the presence or absence of AFP. We would suggest that the Change of αDBP band from homogeneity to heterogeneity may be a sign of carcinogenesis in the body.