人胃粘膜上皮细胞系GES－1的建立及其生物学特性

ESTABLISHMENT AND CHARACTERIZATION OF A SV40 TRANSFORMED HUMAN FETAL GASTRIC EPITHELIAL CELL LINE-GES-1

原代培养的胎儿正常胃粘膜上皮细胞用ＳＶ４０病毒感染，３周左右分离出了转化细胞克隆，并建立了可在体外长期稳定传代的细胞系ＧＥＳ－１。对此细胞的生物学特性分析表明：细胞的基因组中整合了ＳＶ４０Ｔ基因，并在细胞核中表达。染色体为近三倍体（５０～６０之间）。除了转化特性之外，ＧＥＳ－１细胞基本保留了正常的细胞骨架结构（微丝、微管、角蛋白），粘蛋白反应阳性。接种在裸鼠中６个月观察无致瘤性。此细胞系将为深入研究胃上皮细胞癌变提供重要的模式系统。

Abstract 9 month human fetal gastric epithelial cells were primarily cultured and infected with SV40 virus.About 3 weeks after infection the transformed clone named GES-1 were isolated when most ofthe normal cells senesced. One transformed clone named GES-1 were analyzed biologically.The resultsindicated integration of the SV40 T gene and its expression in the cell nuclei and the cells becamehyperploid in chromsomes. This cells also maintained a normal cytoskeleton,positive in PAS reactionand were non-tumorigenic in nude mice.The established cell line may be served as an importantmodel system in study of human gastric carcinogenesis.