宫颈腺癌和鳞癌活检组织中的HPV_（18）和HPV_（16）─—　一项对比研究

USEOF POLYMERASE CHAIN REACTION WITH L1 CONCENSUS PRIMER FOR DETECTION OF HPV 16 ANDHPV18 IN CERVICAL CANCER TISSUES

５０例活检宫颈癌组织，内含２０例宫颈腺癌和２０例宫颈鳞癌，运用ＤｄｅⅠ限制性内切酶，分析Ｌ１区共有引物ＰＣＲ扩增的产物。结果表明，鳞状细胞癌中ＨＰＶ１６的检出率远比腺癌高（８０％，１５％，Ｐ＜０．００１）；相反，腺癌中ＨＰＶ１８的检出率比鳞癌高（４５％，５％，Ｐ＜０．００１）。此差异可能反映了不同的病毒受体存在于具有不同形态潜能的宫颈细胞中，表明特异ＨＰＶ感染在直接致癌中发挥着作用。

Abstract Biopsy materials of cervical carcinoma including 20 cervical adenocarcimomas and 20 squamous cellcarcinomas were collected. A rapid method for determining HPV type was developed, based on DdeⅠrestriction enzymes analysis within the L1 region of HPV, amplified by PCR using consensus primers.The results indicated that HPV type 16 was detected more often in squamous cell carcinomas than in adenocarcinomas (80%vs 15%,P<0.001)conversely, HPV type 18 was detected significantlymore often in adenocarcinoma tissues (45% vs 5%,P<0.001)These differences amy reflect the presence of different virus receptors in cancer cells with different morphologic potential, or, they may indicate that specific HPV infection actually plays a direct role in the process of carcinogenesis.